What in hell IS covid 911 May 2021 edition

anti-barabas-ite

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Escaped True Master

Tom cowan , andy kaufman and stefan lanka discuss Plato, medicine, discernment and germ theory.

If your reality entails the margins of popular consensus, this might be for you.

If you adhere to the "I believe science" paradigm you might want to move along.

Are you a chris hayes, rachel maddow watcher? Or tucker carlson?

Did you get covid11 brain virus and a vax? Or did you maintain optimal health via logos and no vax?

Freewill is available, choose wisely.
 

anti-barabas-ite

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Escaped True Master
From Del Bigtree;


They told us:

  1. Masks are effective.
  2. Covid Vaccines are approved by the FDA.
  3. Covid Vaccines have no negative side effects.
  4. Covid Vaccines have been as tested as every other vaccine.
  5. Shutdowns will keep us healthy.
They were wrong.

ICAN notified New York state and Michigan this week of misinformation regarding the COVID-19 vaccine on their websites, forcing both states to remove the false information from their health websites. New York’s website told citizens that the Covid vaccine has no serious side effects and Michigan told residents that each COVID-19 vaccine had to pass through the same thresholds of research & testing as every other vaccine. On both counts: not true.

And thanks to our actions, both states took down their mis-information.

yes, ICAN has made the CDC remove "vaccines don't cause autism" from their website... not because they admit that they do, rather they can't produce the "SCIENCE" says they don't.

like EMJ is a russian agent trope, jones defense is he can't prove a negative, same thing stanely plotkn will tell you... except there is more proof that vax introduction en mass is detrimental to vulnerable immune systems, and our last 75 years of pharma ag and medicines' have made sure our epigenetics are VULNERABLE, always, and will always need pharmaceuticals!

a very diabolical scheme.
 

anti-barabas-ite

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Escaped True Master
Yes these people would never stand up for a neon nazi like AA as he was "de platformed". this is how it progresses though, nobody spoke up for me and the next thing you know YOU are in the back of the truck at midnight waiting for the bullet in your head....

the cancel culture wars don't stop? until a bigger authoritarian gets ahold of the launch codes? we shall see, until the time of the uprising, watch and repent, gird your loins for the battle and be prepared to die For your Relationship with Jesus Christ.


The war on freedom has reached a fevered pitch. Cancel culture and media censorship are at an all-time high as the rich and powerful continue their crusade to destroy free thought once and for all. At the center of this war is the Center for Countering Digital Hate (CCDH), a group of left-wing mercenaries with the sole purpose of silencing anyone who jeopardizes the financial and political interests of their sponsors.

Earlier this month, we reported that the CCDH had published a “digital hitlist” of the “Top 10 Anti-Vaxxers” which included “The Bollingers” along with 10 others. Apparently, the hatred spewed from the CCDH has affected their ability to perform basic mathematics, such as counting to eleven, because their “top 10” list actually contains 11 names. We’ll just chalk that one up to “vaccine-induced rage.” Since that time, we have been “purged” from Instagram.



The CCDH Agenda
The CCDH is a UK-based group who claims to “disrupt the architecture of online hate and misinformation.” According to their website, they hope to deplatform, demonetize, and demonize what they refer to as “conspiracy theories, misinformation, and hate.”

Within the last week, the CCDH followed up their “Top 10” (or eleven if you can count) list with the results of a survey in which they identify 12 organizations who – they claim – are responsible for nearly two-thirds of “anti-vaccine content.” They’ve cleverly named these groups “the disinformation dozen” and we’re glad to see that they actually have twelve in this list.
disinformation dozen


Despite the globally coordinated release of over 100 mainstream media articles in the span of two days to amplify CCDH’s agenda to target, defame, and deplatform 12 named prominent health freedom advocates, thousands of comments have arisen organically, including over 1,000 comments on the Daily Mail’s front cover article titled, “The ‘disinformation dozen’: Two-thirds of anti-vaxx content circulating on Facebook and Twitter can be traced back to just 12 people including Robert F Kennedy Jr, report claims,” most of which called out the egregiously diversionary and ad hominen tactics being applied through their “report.”

As you can see in the photo to the right, the groups include us (Ty and Charlene), Robert F. Kennedy, Jr., Joseph Mercola, Sherri Tenpenny, Rizza Islam, Rashid Buttar, Erin Elizabeth, Sayer Ji, Kelly Brogan, Christiane Northrup, Ben Tapper, and Kevin Jenkins. We’ve been accused of “spreading disinformation” and “repeatedly” violating Facebook and Twitter’s terms of service. They are currently demanding that the social media platforms demonetize and deplatform us in order to stop what they claim is harmful misinformation about vaccines, COVID-19, and other health-related issues.

But the haters don’t realize that they are only galvanizing our efforts and waking people up in droves! Soon after the “disinformation dozen” article — which received international media amplification across at least 100 different news outlets — was published on March 24th, a tidal wave of social media responses inundated their posts on Instagram, Facebook, and Twitter, calling them out for their clearly hypocritical behavior in viciously attacking and defaming 12 prominent health freedom advocates and whistleblowers.

All 12 of those named in the report have publicly expressed concern over both the US government’s draconian responses to the COVID-19 crisis and the emergency use authorization of experimental mRNA vaccines now being given to the public without informed consent. There are now over 1,500 comments on Facebook alone, expressing anger, disappointment, and a need for accountability from CCDH for their defamatory comments and diversionary tactics.

These attempts to squash free speech and open dialogue are unconstitutional and insulting. They assume that the tens of millions of followers that we have acquired are so daft and uneducated that they are incapable of analyzing information or thinking for themselves. The CCDH wants to ensure that their opinions and agenda are the only ones available in hopes that the American people – and the global population as a whole – will blindly fall in line.

But the Center for Countering Digital Hate is itself a hate group, working as mercenaries for a much greater international agenda. According to a revealing exposé published by Dr. Mercola (hitlist member), the CCDH is part of a campaign by British and American intelligence to eliminate “anti-vaccine propaganda” from public discussion using sophisticated cyberwarfare tools.

Sayer Ji, founder of GreenMedInfo.com and one of the 12 people identified in the report, accurately described the CCDH and their agenda:

The Anti-Vax Industry is a propaganda leaflet with two main objectives. The first is to create a false dichotomy in the public imagination and the second is to build a public-private censorship grid in anticipation of forthcoming government legislation. This is proposed to censor legitimate scientific opinion and evidence-based debate on a wide range of issues the government and its corporate partners would rather silence. Including any questioning of vaccines.

They insist that anyone who has any doubts about any vaccine rejects all vaccines outright. This isn’t true but the CCDH are censors and propagandists, not rationalists.

Comically, they claim they are a non-governmental organization (NGO). While technically plausible, their network of links to government, globalist think tanks and private corporations is extensive.

The CCDH espouses the social reform and political philosophy of progressivism. This advocates alleged progress through the advancement of science, technology and economic development. In the UK this is commonly associated with the political movement found on the right wing of the Labour Party and is the dominant ideology of the Parliamentary Labour Party (PLP).

While still maintaining a putative commitment to representative democracy, it has much in common with the concept of Technocracy and, with the addition of a commitment to maintaining the global dominance of the transatlantic alliance, it is the basis of Blairism. It’s acolytes, such as the CCDH, consider themselves enlightened progressives. However, this sense of elitism produces an intolerance of all opposing views
.”

Which brings us to the first thing you should know about the CCDH…

The Center for Countering Digital Hate is Promoting Digital Hate
Despite the appealing name (who doesn’t want to end digital hate?), the CCDH has done exactly what it claims to stand against. By publishing a digital “hit list” and making blanket statements that demonize people like us, they’ve actually become the hateful extremists that they claim to oppose.

And that isn’t just incendiary rhetoric.

CCDH CEO Imran Ahmed has recently been appointed to the Steering Committee of the UK government’s Commission on Countering Extremism Task Force (CCETF). The UK government’s Commission on Countering Extremism (CCE) 2019 Document called Challenging Hateful Extremism defined, what they call, hateful extremism as follows:

  1. Behaviours that can incite and amplify hate, or engage in persistent hatred, or equivocate about and make the moral case for violence;
  2. And that draw on hateful, hostile or supremacist beliefs directed at an out-group who are perceived as a threat to the wellbeing, survival or success of an in-group;
  3. And that cause, or are likely to cause, harm to individuals, communities or wider society.
Let’s look at the CCDH report and recommendations based on those parameters.

  1. The CCDH has presented these 12 groups as “anti-vaccine propagandists” spreading harmful misinformation. They blame us for vaccine hesitancy (a misleading term for free thinking) and vehemently advocate for our complete destruction as organizations. This has amplified hate, and a quick perusal of the online feedback to the report shows that some people feel there is now a moral justification for violence against us.
  2. The CCDH and their allies have positioned themselves as an authoritative voice of reason that is above reproach. Even questioning the safety and efficacy of vaccines or the measures that governments worldwide have taken since the virus first appeared are considered a major threat to public health and the greater good. They paint organizations like TTAC as greedy liars hell-bent on making a profit while the world burns.
  3. They fundamentally believe that any information that doesn’t align with the narrative that vaccines are safe and effective – or that questions the authoritarian response to the virus – will cause injury and death. They blame us almost exclusively for putting the global community in harm’s way.
It’s clear that the CCDH is itself a group of hateful extremists. They present themselves as unimpeachable authorities while inciting fear and hatred towards specific individuals. If any online rhetoric is likely to incite violence, this is surely it.

This is not only an attack on free speech and open discord, but also a direct affront to the tens of millions of people who choose to follow our work. The CCDH is actively running an massive (and expensive) propaganda campaign in order to silence the leading voices who question their agenda.

On March 25th, a Congressional hearing convened wherein the CEOs of Facebook, Twitter, and Google were interrogated about their role in the ongoing censorship controversial on their platforms. Democratic senator Mike Doyle appeared to bully the CEOs in the clip below, pressuring them in Inquistion-style to both answer YES to the question, “do vaccines work?” and to commit deplatform the 12 “anti-vaxxers” in the CCDH report immediately.




Let’s be honest. Like so many other organizations that are front groups, CCDH is a criminal entity that uses the time-honored strategy of attacking other science based groups that threaten corporate interests by calling them “hate groups”, “anti-vaxxers” or”purveyors of misinformation”. Then when they are called out on the lack of factual basis for these accusations they simply call the unmasker a “conspiracy theorist.”

The CCDH Propaganda Machine
CCDH vaccine propaganda is focused upon polarizing opinion. This fake division is created through CCDH disinformation. Like many propagandists before them, they deal in inaccurate, empty generalizations. They hope to convince their consumers that anyone who ever questions a vaccine must be a nut job. The same dross was recently promulgated by the UK Conservative Prime Minister.

By misleading people that there is no scientific basis for some vaccine skepticism, nor any legitimate concerns about vaccine safety and efficacy, the CCDH are creating fake social divisions in the hope of building real ones. In order to achieve this aim, the CCDH assert that anyone who asks any questions about vaccines is driven by hate and is therefore an extremist who threatens public health, ultimately posing a threat to national security.

They are creating the ludicrous, fake bogeyman of the public health terrorist. The alleged anti-vaxxer as subhuman; a vile, hateful extremist. They are “other.”

This false dichotomy is exactly what fascist regimes have implemented for years. It is a documented logical fallacy in which only two choices are presented when more exist (or a spectrum of possible choices exists between two extremes). False dilemmas are usually characterized by “either this or that” language, but can also be characterized by omissions of choices.

The CCDH would have you believe that you are either for vaccines and absolute totalitarian control, or you are a wild conspiracy theorist who willingly puts the the world at greater risk of harm. Their rhetoric completely eliminates the possibility of open and civil discussion about the scientific risks and benefits of vaccines, chemotherapy, mask mandates, and lockdowns.

This is not only dangerous, but extremely detrimental to a democratic society. When two sides of an argument are so severely polarized, productive conversations are no longer possible. Rather than supporting freedom of speech and open dialogue, the CCDH is willfully fostering an environment in which people must choose a side, with the requirement that each of us actively hate and discredit the other.

When it comes to vaccines, I have no problem identifying as “Anti-Vax.” I believe that the development and testing of vaccines is extremely flawed, and that the risks of most vaccines far outweigh the benefits. But I have always advocated for medical freedom when it comes to care. To insist that others subscribe to your beliefs and agenda is nothing short of tyranny.

The CCDH Will NOT Discuss Vaccine Science
The CCDH are part of the network of so called fact checkers and censors who are using their incredible and seemingly disproportionate influence, suddenly garnered from nowhere, to police opinion on the social media platforms. Unfortunately, their tactic is to completely ignore the available data, demanding that their beliefs be accepted as gospel without question.

Like the government, the CCDH are careful not to mention any of the scientific or historical evidence which questions vaccine efficacy and safety. Instead, they label all who do cite this evidence as radical extremists.

The opening statement in the CCDH’s vaccine propaganda claims:

“Vaccines are one of the most consequential, safe, efficient and effective medical discoveries in history. Few other inventions have saved so many lives.”

Where is their evidence? Just because they state something doesn’t make it true. The truth is that the vast majority of the reduction in mortality rates occurred as a result of broader public health improvements, prior to the widespread use of vaccines.

While the CCDH blithely claims all vaccines are “safe,” they are the only medical discovery where the manufacturers have blanket indemnification against any loss from injury claims. It is not an act of “hate” to ask why this needs to be the case if they are so safe.

The CCDH states that anyone who asks such questions has fringe and extremist views. They claim consideration of vaccine safety and efficacy should not be permitted and sharing any information or evidence which questions vaccines should be banned. For example, they deny people’s right to know any of the information we are about to discuss. They claim it is all hate driven disinformation which presents a threat to national security.

When trialing a vaccine, inoculated animal test subjects can be deliberately exposed to the targeted virus in a challenge trial. The results from challenge trials have blighted all previous attempts to develop a SARS-CoV vaccine.

While the test subjects developed the hoped for antibodies and proteins, when they were challenged with the virus their immune systems were found to be hypersensitive. This induced life threatening illness and caused a range of serious health conditions.

The interferon gamma (IFN-y) induced protein IP10, encoded in humans by the CXCL10 gene, is thought to be a possible cause of the cytokine storm which leads to Acute Respiratory Distress Syndrome (ARDS). Persistent high levels of IP10 send the immune system into overdrive. Much of the immunopathological damage sustained by a small minority of SARS infected patients is thought to arise as a consequence of interferon gamma (IFN-y) related cytokine storms. Italian researchers noted:

“Accumulating studies indicated that the cytokine storm caused by SARS is mainly related to IL-1β, IL-6, IL12A, IFN-γ, IP10 and MCP1, and the cytokine storm caused by MERS is mainly related to IFNγ, TNFα, IL15 and IL17A.”

It is therefore somewhat concerning that during the challenge trials for the ChAdOx1 nCoV-19 (AZD1222 SARS-CoV-2) vaccine, currently being developed in by AstraZeneca and Oxford University, the following was noted:

“Cytokines in serum were analysed after challenge to monitor immune responses. We observed an upregulation [increased cellular response] in IFN-γ at 1 DPI in ChAdOx1 nCoV-19 vaccinated animals, but not in control animals.”

While an upregulation in IFN-y potentially has both beneficial and harmful inflammatory effects, we don’t know what the long term IP10 (CXCL10) levels for inoculated test subjects were because it wasn’t investigated in the vaccine trials. However, an upregulation in IFN-y suggests the possibility of the overexpression of the potentially lethal cytokine storm inducing CXCL10.

The ChAdOx1 nCoV-19 study only checked IFN-y upregulation for one day post inoculation (1 DPI). Then again, this really isn’t of any concern for the multinational corporations, like AstraZeneca, who make vaccines. As usual, they have been given immunity from prosecution. They have no liability for vaccine injury, and therefore have everything to gain and absolutely nothing to lose.

Having found these results in all of the 6 macaque monkeys they inoculated, the AstraZeneca-Oxford team felt this was nothing to worry about and went ahead with large scale human trials. The results of these trials raised further reason for concern.

Contrary to the claims made by the mainstream media (MSM), this was not a randomized double blind placebo controlled trial (RCT). Instead of an inert placebo, the human test subjects were either given the AstraZeneca-Oxford vaccine or the MenACWY vaccine.

The possible side effects of the MenACWY vaccine include headaches, nausea, fever, elevated heart rate, loss of consciousness, paralysis and seizures. Using the MenACWY vaccine as your control, to measure relative safety, will probably provide a favorable safety profile providing the recipient of your new vaccine doesn’t immediately drop dead the moment you inoculate them.

The people who were selected for the AstraZeneca-Oxford Phase 1 and initial Phase 2 trial were all in good health and aged between 18 – 55 years. The median age of the participants was 35 yrs. The average age for those requiring COVID 19 hospital treatment is at least 60 years.

COVID 19 risks increase appreciably with age. In the UK, more than 89% of those who have died “with” COVID 19 were over 65 years old.

The UK government have announced their intention to initially vaccinate those in the at risk group and front line key workers. These are primarily older people with serious comorbidity.

With the exception of younger key workers, the initial phases of the trials didn’t test the vaccine with the demographic who will be the first to receive it. While the trials have now been expanded to include some older people and children, early results indicate the need for considerable caution.

Of the vaccinated group 70% reported fatigue, 68% headaches, 60% had muscle pain and more than 50% ran a fever. In addition, 9% reported temperatures of at least 38°C and an alarming 1% reported a high fever of more than 39°C.

While researchers stated that these adverse reaction were “well tolerated,” by the relatively young and healthy test subjects, the same cannot simply be assumed for older at risk groups.

These adverse reactions present a far greater health risk to the most vulnerable in society. The demographic which the vaccine is supposed to protect.

These early trial outcomes have been met with universal, uncritical praise by the UK MSM because the AstraZeneca-Oxford vaccine did stimulate an immune response. However, evidence is now emerging that up to 60% of the population may already have general immunity. If this is the case, the relative benefit to vulnerable people, in light of the adverse reactions, would appear questionable.

According to the CCDH, none of these concerns have any basis in either fact or science. Anyone raising these concerns is now labeled as a radical extremist and a threat to global health.

But it’s not just the 12 of us who are under attack. If you follow TTAC, GreenMedInfo, Dr. Mercola, Robert F. Kennedy, Jr. and the Children’s Health Defense Fund, or the other leading voices in the natural health space, CCDH believes that you are too STUPID to think for yourself.

The CCDH Thinks You’re an Idiot
At first glance, the CCDH report seems to attack only the 12 groups mentioned above. But their cries for us to be silenced tell a different narrative. If removing us from online social platforms is truly the solution to ending “misinformation” and “vaccine hesitancy,” it follows that our 60 million+ followers are unintelligent simpletons who will simply shift their thinking to agree with whatever narrative they’re served.

This is a mistake that fascist regimes have repeatedly made throughout history. The assumption that silencing free speech will also silence free thought is incorrect – and insulting. The communist regime in China has been trying (unsuccessfully) for years to silence free thought by controlling media outlets, the internet, and even the ability to travel freely. In Hong Kong, this oppression has become violent, with democratic leaders rounded up in the middle of the night and detained while citizens yearning for freedom face extreme censorship, curfews, and military rule.

Science-Based Publishing
Imagine if TTAC published an article claiming that pigs could fly. Would our readers blindly assume that it was true? OF COURSE NOT! That allegation is ludacris and has no foundation in science or reason. But the information that we share is based on tangible data, presented in a way that allows our readers to make informed decisions for themselves.

When we say that masks are ineffective against COVID-19 and can lead to health problems, we back it up.

Research published in the Annals of Internal Medicine at the first of April indicated that “both surgical and cotton masks seem to be ineffective in preventing the dissemination of SARS–CoV-2 from the coughs of patients with COVID-19.”

A study from May of last year found that 81% of healthcare workers developed headaches after wearing masks.

Even the label on boxes of surgical masks warn that “THIS PRODUCT WILL NOT PROVIDE ANY PROTECTION AGAINST COVID-19 (CORONAVIRUS) OR OTHER VIRUSES OR CONTAMINANTS.”

When we publish articles expressing concern for the unconstitutional (and, in some cases, anti-Semitic) mandates that New York City Mayor Bill DeBlasio attempted to force regarding the MMR vaccine, we support our argument with facts.

Did you know that From 1959-1962 – just before the vaccine was introduced – measles was fatal in only 0.01% of cases? And while the number of unvaccinated children has quadrupled since 2001, from 2000-2016 measles deaths decreased by 84%.

Or that malnutrition, especially vitamin A deficiency, is a primary cause of about 90,000 measles deaths annually in underdeveloped nations? In the U.S. and other developed countries, up to 92% of hospitalized measles patients are low in vitamin A.

How about the fact that measles, stroke, Guillain-Barré syndrome, and death are all listed as possible side effects on the package insert for Merck’s ProQuad vaccine?

Since the turn of the millennium, Americans are 84 times more likely to die from contact with a powered lawnmower than from measles. Yet state and local governments continue to pass legislation forcing parents to vaccinate their children… legislation that generates almost $2 BILLION in annual revenue for companies like Merck on the MMR vaccine alone.

But these aren’t discussions that the CCDH is willing to have. Rather than address our concerns (or the research that supports them), these self-proclaimed authorities choose to label us as lying extremists – despite published research and data to the contrary.

The bottom line?

The CCDH doesn’t believe that you are capable of independent thought or critical thinking. They truly believe that each and every one of you are mindless nitwits who have been tricked into believing us because you were unfortunate enough to stumble across our work. They believe that if they can simply change the proverbial channel, you will immediately change your way of thinking to align with their own.

And there’s one more major issue.

Execution by Censorship
By restricting access to this information, the CCDH and their allies are contributing to the death and detriment of hundreds of thousands around the globe. When we speak out against untested vaccines, unconstitutional lockdowns, or the bullied approach to cancer treatment prominent in most hospitals, we’re doing it to help save lives.

Vaccines
The Vaccine Adverse Event Reporting System, or VAERS, has documented over 2,500 deaths in recipients of the U.S. COVID-19 vaccines. They’ve reported 559,000 deaths from the virus itself. This amounts to a .002% mortality rate from the vaccine and a .17% population mortality rate from the virus. But there are some major questions regarding those numbers.

VAERS is a passive monitoring system, meaning doctors are not required to report adverse events. Several studies have suggested that as few as 5% of all adverse reactions are reported. If that’s true, the mortality rate for the vaccine may be closer to .04%, or 1 in 2,500.

Conversely, reporting for COVID-19 deaths has been wildly unreliable. While the reported death rate in the U.S. is .17%, the global tally is closer to .04%. The most likely explanation for the high mortality rate in the U.S. is simple: false reporting. According to recent data from the CDC, only 6% of deaths attributed to COVID-19 can actually be attributed to the virus. The other 94% were accompanied by other comorbidities, such as late-stage cancer, immune disease, and injuries.

If a person dies and tests positive for the virus, they’re added to the body count. However, that’s scientifically irresponsible at best and criminally negligent at the worst. There have been reports of people around the country dying from car accidents, drowning, or premature birth who have been counted among the coronavirus victims.

As infection, hospitalization, and deaths continue to go down (likely because most of the population has already been exposed), the push to vaccinate every man, woman, and child is aggressively underway. Our concerns about the safety of the vaccines, along with the threat of our government forcing us to receive it, are not unwarranted.

Lockdowns
One of the most common attacks we’ve heard on those who support reopening our economy and salvaging our liberties is that we care more about the economy than human life. But the truth is that the two are invariably intertwined.

The more damage we do to our society and economy, the higher the death toll will rise. In fact, there will almost certainly be more loss of life from the reaction to coronavirus than the disease itself. Reuters summarized a few of them beautifully:

Domestic Violence
Trapped at home with their abusers, some domestic violence victims are already experiencing more frequent and extreme violence, said Katie Ray-Jones, the chief executive officer of the National Domestic Violence Hotline.

Domestic violence programs across the country have cited increases in calls for help, news accounts reported – from Cincinnati to Nashville, Portland, Salt Lake City and statewide in Virginia and Arizona. The YWCA of Northern New Jersey, in another example, told Reuters its domestic violence calls have risen up to 24%.

“There are special populations that are going to have impacts that go way beyond COVID-19,” said Ray-Jones, citing domestic violence victims as one.

Vulnerable Students
Students, parents, and teachers all face challenges adjusting to remote learning, as schools nationwide have been closed and online learning has begun.

Some experts are concerned that students at home, especially those living in unstable environments or poverty, will miss more assignments. High school students who miss at least three days a month are seven times more likely to drop out before graduating and, as a result, live nine years less than their peers, according to a Robert Wood Johnson Foundation report.

Among the most vulnerable: the more than 6 million special education students across the United States. Without rigorous schooling and therapy, these students face a lifetime of challenges.

Special needs students “benefit the most from highly structured and customized special education,” said Sharon Vaughn, executive director of the The Meadows Center for Preventing Educational Risk at the University of Texas. “This means that they are the group that are most likely to be significantly impacted by not attending school both in the short and long term.”

In New Jersey, Matawan’s Megan Gutierrez has been overwhelmed with teaching and therapy duties for her two nonverbal autistic sons, eight and 10. She’s worried the boys, who normally work with a team of therapists and teachers, will regress. “For me, keeping those communications skills is huge, because if they don’t, that can lead to behavioral issues where they get frustrated because they can’t communicate,” Gutierrez said.

Soaring Suicides
In Europe and the United States, suicide rates rise about 1% for every one percentage point increase in unemployment, according to research published by lead author Aaron Reeves from Oxford University. During the last recession, when the unemployment in the United States peaked at 10%, the suicide rate jumped, resulting in 4,750 more deaths. If the unemployment rate increases to 20%, the toll could well rise.

“Sadly, I think there is a good chance we could see twice as many suicides over the next 24 months than we saw during the early part of the last recession,” Reeves told Reuters. That would be about 20,000 additional dead by suicide in the United States and Europe.

Less than three weeks after extreme suppression measures began in the United States, unemployment claims rose by nearly 10 million. Treasury Secretary Steven Mnuchin warned the rate could reach 20% and Federal Reserve economists predicted as high as 32%. Europe faces similarly dire forecasts.

Some researchers caution that suicide rates might not spike so high. The conventional wisdom is that more people will kill themselves amid skyrocketing unemployment, but communities could rally around a national effort to defeat COVID-19 and the rates may not rise, said Anne Case, who researches health economics at Princeton University. “Suicide is hard to predict even in the absence of a crisis of Biblical proportions,” Case said.

This week, the Air Force Academy in Colorado Springs, Colorado, relaxed its strict social isolation policies after the apparent suicides of two cadet seniors in late March, The Gazette, a Colorado Springs newspaper, reported. While juniors, sophomores and freshmen had been sent home, the college seniors were kept isolated in dorms, and some had complained of a prison-like setting. Now, the seniors will be able to leave campus for drive-thru food and congregate in small groups per state guidelines.

Public Health Crippled
Local health departments run programs that treat chronic diseases such as diabetes. They also help prevent childhood lead poisoning and stem the spread of the flu, tuberculosis and rabies. A severe loss of property and sales tax revenue following a wave of business failures will likely cripple these health departments, said Adriane Casalotti, chief of government affairs with the National Association of County and City Health Officials, a nonprofit focused on public health.

After the 2008 recession, local health departments in the U.S. lost 23,000 positions as more than half experienced budget cuts. While it’s become popular to warn against placing economic concerns over health, Casalotti said that, on the front lines of public health, the two are inexorably linked. “What are you going to do when you have no tax base to pull from?” she asked.

Carol Moehrle, director of a public health department that serves five counties in northern Idaho, said her office lost about 40 of its 90 employees amid the last recession. The department had to cut a family planning program that provided birth control to women below the poverty line and a program that tested for and treated sexually transmitted diseases. She worries a depression will cause more harm.

“I honestly don’t think we could be much leaner and still be viable, which is a scary thing to think about,” Moehrle said.

Job-loss Mortality
Rises in unemployment during large recessions can set in motion a domino effect of reduced income, additional stress and unhealthy lifestyles. Those setbacks in income and health often mean people die earlier, said Till von Wachter, a University of California Los Angeles professor who researches the impact of job loss. Von Wachter said his research of past surges in unemployment suggests displaced workers could lose, on average, a year and a half of lifespan. If the jobless rate rises to 20%, this could translate into 48 million years of lost human life.

Von Wachter cites measures he believes could mitigate the effects of unemployment. The Coronavirus Aid, Relief, and Economic Security Act approved by the White House last week includes emergency loans to businesses and a short-time compensation program that could encourage employers to keep employees on the payroll.

Young People Suffer
Young adults entering the job market during the coronavirus suppression may pay an especially high price over the long term. First-time job hunters seeking work during periods of high unemployment live shorter and unhealthier lives, research shows. An extended freeze of the economy could shorten the lifespan of 6.4 million Americans entering the job market by an average of about two years, said Hannes Schwandt, a health economics researcher at Northwestern University, who conducted the study with von Wachter. This would be 12.8 million years of life lost.

Thousands of college graduates will enter a job market at a time global business is frozen. Jason Gustave, a senior at William Paterson University in New Jersey who will be the first in his family to graduate from college, had a job in physical therapy lined up. Now his licensure exam is postponed and the earliest he could start work is September.

“It all depends on where the economy goes,” he said. “Is there a position still available?”

Even the U.N., which has vigorously supported the draconian suspension of industry, society, and freedom, says that “hundreds of thousands of children could die this year due to the global economic downturn sparked by the coronavirus pandemic and tens of millions more could fall into extreme poverty as a result of the crisis”

Our opposition to the lockdowns is not “misinformation.” It stems from genuine, reasonable concern for the loss of life and long-term damage to public welfare resulting from the lockdowns. In July of last year, the Associated Press reported that virus-linked hunger resulting from the economic shutdown was responsible for 10,000 child deaths each month.

But these are things the CCDH isn’t willing to discuss.

These are liars and bullies who choose to ignore opinions – and facts – that don’t fit their narrative. They want to stifle our fundamental rights to free speech and thought and believe that YOU are too stupid to think for yourself.

Everyone, including the CCDH, has the right to express their views and partake in robust and open debate. However, the CCDH’s supremacist beliefs render them incapable of doing so, as they cannot tolerate anything which contradicts or challenges their ideology and objectives. The question we should be asking is:

WHY?
Why would an organization spend so much time and money to silence us? How did the group become so prominent and so well-funded in just over 2 years? What is the real motivation for so aggressively and tirelessly working to silence anyone who raises legitimate questions about the safety and efficacy of vaccines?

Because the CCDH and it’s partners are a corrupt group of mercenaries funded by the global elite for the sole purpose of pushing a new globalist agenda while ushering in an historic era of censorship and despotism.

In Part 2, we’ll uncover the CCDH’s links to governments, think tanks, and other special interest groups. Our society is barreling towards a technocratic dystopia, with groups like the CCDH leading the vanguard. We’ll look at how we got here, what to expect next, and why this war is far from over.



CALL TO ACTION

  1. Please join us in responding to the CCDH’s piece on their Instagram, Facebook, and Twitter in order to hold them accountable and call them out on their HATE.
  2. Join our partner organization, United Medical Freedom PAC, which advocates for your God-given rights in the face of an increasingly tyrannical agenda intent on stripping you of all your freedoms, and consider making a one time donation.
  3. Please join us on our Telegram channel and join a CENSORSHIP FREE discussion about all the things that may get you kicked off the other social media outlets – like vaccine truth, Cov1D, natural cancer remedies, and “unallowed” political views.
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PANDA (Pandemics – data and analysis) has been outspoken with regards to the policy-makers' reaction to Covid-19, lockdowns and other approaches to the virus. Its viewpoints have ruffled feathers over the past year, with many in the establishment openly hostile towards the group of actuaries, accountants, economists and other professionals who participate in the global think tank. Nick Hudson, co-founder of PANDA, spoke at the inaugural BizNews Investment Conference in March 2021. Here's his keynote address.
 

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ISSN: 1080-6059


Volume 26, Number 6—June 2020
Research

Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States
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Jennifer Harcourt1, Azaibi Tamin1, Xiaoyan Lu, Shifaq Kamili, Senthil K. Sakthivel, Janna Murray, Krista Queen, Ying Tao, Clinton R. Paden, Jing Zhang, Yan Li, Anna Uehara, Haibin Wang, Cynthia Goldsmith, Hannah A. Bullock, Lijuan Wang, Brett Whitaker, Brian Lynch, Rashi Gautam, Craig Schindewolf, Kumari G. Lokugamage, Dionna Scharton, Jessica A. Plante, Divya Mirchandani, Steven G. Widen, Krishna Narayanan, Shinji Makino, Thomas G. Ksiazek, Kenneth S. Plante, Scott C. Weaver, Stephen Lindstrom, Suxiang Tong, Vineet D. Menachery2, and Natalie J. Thornburg2Comments to Author
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Harcourt, A. Tamin, X. Lu, K. Queen, Y. Tao, C.R. Paden, Y. Li, C. Goldsmith, B. Whitaker, R. Gautam, S. Lindstrom, S. Tong, N.J. Thornburg); Eagle Medical Services, Atlanta (S. Kamili, S.K. Sakthivel, J. Murray, B. Lynch); IHRC, Atlanta (J. Zhang, H. Wang); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (A. Uehara); Synergy America, Inc., Atlanta (H.A. Bullock, L. Wang); University of Texas Medical Branch, Galveston, Texas, USA (C. Schindewolf, K.G. Lokugamage, D. Mirchandani, S. Widen, K. Narayanan, S. Makino, T.G. Ksiazek, S.C. Weaver, V.D. Menachery); World Reference Center for Emerging Viruses and Arboviruses, Galveston (D. Scharton, J.A. Plante, T.G. Ksiazek, K.S. Plante, S.C. Weaver, V.D. Menachery)
Cite This Article

Abstract
The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.
A novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been identified as the source of a pneumonia outbreak in Wuhan, China, in late 2019 (1,2). The virus was found to be a member of the β coronavirus family, in the same species as SARS-CoV and SARS-related bat CoVs (3,4). Patterns of spread indicate that SARS-CoV-2 can be transmitted person-to-person, and may be more transmissible than SARS-CoV (57). The spike protein of coronaviruses mediates virus binding and cell entry. Initial characterization of SARS-CoV-2 spike indicates that it binds the same receptor as SARS-CoV angiotensin-converting enzyme, which is expressed in both upper and lower human respiratory tracts (8).
The unprecedented rapidity of spread of this outbreak represents a critical need for reference reagents. The public health community requires viral lysates to serve as diagnostic references, and the research community needs virus isolates to test antiviral compounds, develop new vaccines, and perform basic research. In this article, we describe isolation of SARS-CoV-2 from a patient who had coronavirus disease (COVID-19) in the United States and described its genomic sequence and replication characteristics. We have made the virus isolate available to the public health community by depositing it into 2 virus reagent repositories.
Methods
Specimen Collection
Virus isolation from patient samples was deemed not to be human subjects research by the National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention (CDC) (research determination no. 0900f3eb81ab4b6e). Clinical specimens from a case-patient who had acquired COVID-19 during travel to China and who was identified in Washington, USA, were collected as described (1). Nasopharyngeal (NP) and oropharyngeal (OP) swab specimens were collected on day 3 postsymptom onset, placed in 2–3 mL of viral transport medium, used for molecular diagnosis, and frozen. Confirmed PCR-positive specimens were aliquoted and refrozen until virus isolation was initiated.
Cell Culture, Limiting Dilution, and Virus Isolation
We used Vero CCL-81 cells for isolation and initial passage. We cultured Vero E6, Vero CCL-81, HUH 7.0, 293T, A549, and EFKB3 cells in Dulbecco minimal essential medium (DMEM) supplemented with heat-inactivated fetal bovine serum (5% or 10%) and antibiotics/antimycotics (GIBCO, https://www.thermofisher.comExternal Link). We used both NP and OP swab specimens for virus isolation. For isolation, limiting dilution, and passage 1 of the virus, we pipetted 50 μL of serum-free DMEM into columns 2–12 of a 96-well tissue culture plate, then pipetted 100 μL of clinical specimens into column 1 and serially diluted 2-fold across the plate. We then trypsinized and resuspended Vero cells in DMEM containing 10% fetal bovine serum, 2× penicillin/streptomycin, 2× antibiotics/antimycotics, and 2× amphotericin B at a concentration of 2.5 × 105 cells/mL. We added 100 μL of cell suspension directly to the clinical specimen dilutions and mixed gently by pipetting. We then grew the inoculated cultures in a humidified 37°C incubator in an atmosphere of 5% CO2 and observed for cytopathic effects (CPEs) daily. We used standard plaque assays for SARS-CoV-2, which were based on SARS-CoV and Middle East respiratory syndrome coronavirus (MERS-CoV) protocols (9,10).
When CPEs were observed, we scraped cell monolayers with the back of a pipette tip. We used 50 μL of viral lysate for total nucleic acid extraction for confirmatory testing and sequencing. We also used 50 μL of virus lysate to inoculate a well of a 90% confluent 24-well plate.
Inclusivity/Exclusivity Testing
From the wells in which CPEs were observed, we performed confirmatory testing by using real-time reverse transcription PCR (CDC) and full-genome sequencing (1). The CDC molecular diagnostic assay targets 3 portions of the nucleocapsid gene, and results for all 3 portions must be positive for a sample to be considered positive (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-detection-instructions.html and https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html). To confirm that no other respiratory viruses were present, we performed Fast Track Respiratory Pathogens 33 Testing (FTD Diagnostics, http://www.fast-trackdiagnostics.comExternal Link).
Whole-Genome Sequencing
We designed 37 pairs of nested PCRs spanning the genome on the basis of the coronavirus reference sequence (GenBank accession no. NC045512). We extracted nucleic acid from isolates and amplified by using the 37 individual nested PCRs. We used positive PCR amplicons individually for subsequent Sanger sequencing and also pooled them for library preparation by using a ligation sequencing kit (Oxford Nanopore Technologies, https://nanoporetech.comExternal Link), subsequently for Oxford Nanopore MinION sequencing. We generated consensus nanopore sequences by using Minimap version 2.17 (https://github.comExternal Link) and Samtools version 1.9 (http://www.htslib.orgExternal Link). We generated consensus sequences by Sanger sequencing from both directions by using Sequencher version 5.4.6 (https://www.genecodes.comExternal Link), and further confirmed them by using consensus sequences generated from nanopore sequencing.
To sequence passage 4 stock, we prepared libraries for sequencing by using the Next Ultra II RNA Prep Kit (New England Biolabs, https://www.neb.comExternal Link) according to the manufacturer’s protocol. In brief, we fragmented ≈70–100 ng of RNA for 15 min, followed by cDNA synthesis, end repair, and adaptor ligation. After 6 rounds of PCR, we analyzed libraries by using an Agilent Bioanalyzer (https://www.agilent.comExternal Link) and quantified them by using a quantitative PCR. We pooled samples and sequenced samples by using a paired-end 75-base protocol on an Illumina (Illumina, Inc., https://www.illumina.comExternal Link) MiniSeq instrument and using the High-Output Kit and then processed reads by using Trimmomatic version 0.36 (11) to remove low-quality base calls and any adaptor sequences. We used the de novo assembly program ABySS (12) to assemble the reads into contigs by using several different sets of reads and kmer values ranging from 20 to 40. We compared contigs >400 bases against the National Center for Biotechnology Information (Bethesda, MD, USA) nucleotide collection using BLAST (https://blast.ncbi.nlm.nih.govExternal Link). A nearly full-length viral contig obtained in each sample had 100% identity to the 2019-nCoV/USA-WA1/2020 strain (GenBank accession no. MN985325.1). All the remaining contigs mapped to either host cell rRNA or mitochondria. We mapped the trimmed reads to the reference sequence by using BWA version 0.7.17 (13) and visualized these reads by using the Integrated Genomics Viewer (14) to confirm the identity with the USA-WA1/2020 strain.
Electron Microscopy
We scraped infected Vero cells from the flask, pelleted by low-speed centrifugation, rinsed with 0.1 mol/L phosphate buffer, pelleted again, and fixed for 2 h in 2.5% buffered glutaraldehyde. We then postfixed specimens with 1% osmium tetroxide, en bloc stained with 4% uranyl acetate, dehydrated, and embedded in epoxy resin. We cut ultrathin sections, stained them with 4% uranyl acetate and lead citrate, and examined them by using a Thermo Fisher/FEI Tecnai Spirit electron microscope (https://www.fei.comExternal Link).
Protein Analysis and Western Blotting
We harvested cell lysates by using Laemmli sodium dodecyl sulfate–polyacrylamide gel electrophoresis sample buffer (Bio-Rad, https://www.bio-rad.comExternal Link) containing 2% SDS and 5% β-mercaptoethanol. We removed the cell lysates from a Biosafety Level 3 Laboratory, boiled them, and load them onto a polyacrylamide gel. We subjected the lysates to sodium dodecyl sulfate–polyacrylamide gel electrophoresis, followed by transfer to a polyvinylidene difluoride polyvinylidene fluoride membrane. We then blocked the membrane in 5% nonfat dry milk dissolved in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h, followed by a short wash with TBS-T. We incubated the membrane overnight with primary antibody, either rabbit polyclonal serum against the SARS-CoV spike protein (#40150-T52; Sino Biological, https://www.sinobiological.comExternal Link), β-actin antibody (#4970; Cell Signaling Technology, https://www.cellsignal.comExternal Link), or a custom rabbit polyclonal serum against SARS-CoV nucleocapsid. We then washed the membrane with 3 times with TBS-T and applied horseradish peroxidase-conjugated secondary antibody for 1 h. Subsequently, we washed the membrane 3 times with TBS-T, incubated with Clarity Western ECL Substrate (#1705060S; Bio-Rad), and imaged with a multipurpose imaging system.
Generation of SARS-CoV Nucleocapsid Antibodies
We used the plasmid pBM302 (15) to express SARS-CoV nucleocapsid protein, with a C-terminal His6 tag, to high levels within the inclusion bodies of Escherichia coli and the recombinant protein was purified from the inclusion bodies by using nickel-affinity column chromatography under denaturing conditions. We used stepwise dialysis against Tris/phosphate buffer to refold the recombinant SARS-CoV nucleocapsid protein with decreasing concentrations of urea to renature the protein. We then immunized rabbits with the renatured, full-length, SARS-CoV nucleocapsid protein to generate an affinity-purified rabbit anti–SARS-CoV nucleocapsid protein polyclonal antibody.
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Results
Thumbnail of Cytopathic effect caused by severe acute respiratory syndrome coronavirus 2 from patient with 2019 novel coronavirus disease, United States, 2020. A–C) Phase-contrast microscopy of Vero cell monolayers at 3 days postinoculation: A) Mock, B) nasopharyngeal specimen, C) oropharyngeal specimen. Original magnifications ×10). D) Electron microscopy of virus isolate showing extracellular spherical particles with cross-sections through the nucleocapsids (black dots). Arrow indicates a coro

Figure 1. Cytopathic effect caused by severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States, 2020. A–C) Phase-contrast microscopy of Vero cell monolayers at 3 days postinoculation: A) Mock,...
A patient was identified with confirmed COVID-19 in Washington State on January 22, 2020. CPE was not observed in mock infected cells (Figure 1, panel A). Cycle threshold (Ct) values were 18–20 for NP specimens and 21–22 for OP specimens (1). The positive clinical specimens were aliquoted and refrozen inoculated into cell culture on January 22, 2020. We observed CPE 2 days postinoculation and harvested viral lysate on day 3 postinoculation (Figure 1, panels B, C). We used 50 μL of passage 1 viral lysates for nucleic acid extraction to confirm the presence of SARS-CoV-2 by using the CDC molecular diagnostic assay (1). The Ctvalues of 3 nucleic acid extractions were 16.0–17.1 for nucleocapsid portion 1, 15.9–17.1 for nucleocapsid portion 2, and 16.2–17.3 for nucleocapsid portion 3, which confirmed isolation of SARS-CoV-2 (Ct <40 is considered a positive result). We also tested extracts for 33 additional different respiratory pathogens by using the Fast Track 33 Assay. No other pathogens were detected. Identity was additionally supported by thin-section electron microscopy (Figure 1, panel D). We observed a morphology and morphogenesis characteristic of coronaviruses.
We used isolates from the first passage of an OP and an NP specimen for whole-genome sequencing. The genomes from the NP specimen (GenBank accession MT020880) and OP specimen (GenBank accession no. MT020881) showed 100% identity with each other. The isolates also showed 100% identity with the corresponding clinical specimen (GenBank accession no. MN985325).
After the second passage, we did not culture OP and NP specimens separately. We passaged virus isolate 2 more times in Vero CCL-81 cells and titrated by determining the 50% tissue culture infectious dose (TCID50). Titers were 8.65 × 106 TCID50/mL for the third passage and 7.65 × 106 TCID50/mL for the fourth passage.
We passaged this virus in the absence of trypsin. The spike protein sequence of SARS-CoV-2 has an RRAR insertion at the S1-S2 interface that might be cleaved by furin (16). Highly pathogenic avian influenza viruses have highly basic furin cleavage sites at the hemagglutinin protein HA1-HA2 interface that permit intracellular maturation of virions and more efficient viral replication (17). The RRAR insertion in SARS-CoV-2 might serve a similar function.
Thumbnail of Viral propagation and quantitation of severe acute respiratory syndrome coronavirus 2 from patient with 2019 novel coronavirus disease, United States, 2020. A) Two virus passage 4 stocks (black and gray circles) were quantified by using plaque assay at day 2 (solid circles) and day 3 (open circles) postinfection of Vero E6 and Vero CCL81 cells. B) Plaque morphology for virus on Vero E6 and Vero CCL81 at day 2 and day 3 postinoculation. C) Cell monolayers 2 days postinfection of Vero

Figure 2. Viral propagation and quantitation of severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States, 2020. A) Two virus passage 4 stocks (black and gray circles) were quantified...
We subsequently generated a fourth passage stock of SARS-CoV-2 on VeroE6 cells, another fetal rhesus monkey kidney cell line. We sequenced viral RNA from SARS-CoV-2 passage 4 stock and confirmed it to have no nucleotide mutations compared with the original reference sequence (GenBank accession no. MN985325). SARS-CoV has been found to grow well on VeroE6 cells and MERS-CoV on Vero CCL81 cells (18,19). To establish a plaque assay and determine the preferred Vero cell type for quantification, we titered our passage 4 stock on VeroE6 and VeroCCL81 cells. After infection with a dilution series, SARS-CoV-2 replicated in both Vero cell types; however, the viral titers were slightly higher in VeroE6 cells than in Vero CCL81 cells (Figure 2, panel A). In addition, plaques were more distinct and visible on Vero E6 cells (Figure 2, panel B). As early as 2 days postinoculation, VeroE6 cells produced distinct plaques visible by staining with neutral red. In contrast, Vero CCL81 cells produced less clear plaques and was most easily quantitated by staining with neutral red 3 days postinoculation. On the individual plaque monolayers, SARS-CoV-2 infection of Vero E6 cells produced CPE with areas of cell clearance (Figure 2, panel C). In contrast, Vero CCL81 cells had areas of dead cells that had fused to form plaques, but the cells did not clear. Together, these results suggest that VeroE6 cells might be the best choice for amplification and quantification, but both Vero cell types support amplification and replication of SARS-CoV-2.
Thumbnail of Cell lines from patient with coronavirus disease, United States, 2020, susceptible to SARS coronavirus 2 (SARS-CoV-2). Cell lines were infected with a high multiplicity of infection (>5), washed after adsorption, and subsequently harvested 24 h postinfection for viral titer and protein lysates. A) Viral titer for SARS-CoV-2 quantitated by plaque assay on Vero E6 cells 2 days postinoculation. Infected cell protein lysates were probed by using Western blotting with B) rabbit polycl

Figure 3. Cell lines from patient with coronavirus disease, United States, 2020, susceptible to SARS coronavirus 2 (SARS-CoV-2). Cell lines were infected with a high multiplicity of infection (>5), washed after adsorption, and...
Because research has been initiated to study and respond to SARS-CoV-2, information about cell lines and types susceptible to infection is needed. Therefore, we examined the capacity of SARS-CoV-2 to infect and replicate in several common primate and human cell lines, including human adenocarcinoma cells (A549), human liver cells (HUH7.0), and human embryonic kidney cells (HEK-293T), in addition to Vero E6 and Vero CCL81 cells. We also examined an available big brown bat kidney cell line (EFK3B) for SARS-CoV-2 replication capacity. Each cell line was inoculated at high multiplicity of infection and examined 24 h postinfection (Figure 3, panel A). No CPE was observed in any of the cell lines except in Vero cells, which grew to >107 PFU at 24 h postinfection. In contrast, HUH7.0 and 293T cells showed only modest viral replication, and A549 cells were incompatible with SARS-CoV-2 infection. These results are consistent with previous susceptibility findings for SARS-CoV and suggest other common culture systems, including MDCK, HeLa, HEP-2, MRC-5 cells, and embryonated eggs, are unlikely to support SARS-CoV-2 replication (2022). In addition, SARS-CoV-2 did not replicate in bat EFK3B cells, which are susceptible to MERS-CoV. Together, the results indicate that SARS-CoV-2 maintains a similar profile to SARS-CoV in terms of susceptible cell lines.
Having established robust infection with SARS-CoV-2 in several cell types, we next evaluated the cross-reactivity of SARS-CoV antibodies against the SARS-CoV-2. Cell lysates from infected cell lines were probed for protein analysis; we found that polyclonal serum against the SARS-CoV spike protein and nucleocapsid proteins recognize SARS-CoV-2 (Figure 3, panels B, C). The nucleocapsid protein, which is highly conserved across the group 2B family, retains >90% amino acid identity between SARS-CoV and SARS-CoV-2. Consistent with the replication results (Figure 3, panel A), SARS-CoV-2 showed robust nucleocapsid protein in both Vero cell types, less protein in HUH7.0 and 293T cells, and minimal protein in A549 and EFK3B cells (Figure 3, panel B). The SARS-CoV spike protein antibody also recognized SARS-CoV-2 spike protein, indicating cross-reactivity (Figure 3, panel C). Consistent with SARS CoV, several cleaved and uncleaved forms of the SARS-CoV-2 spike protein were observed. The cleavage pattern of the SARS spike positive control from Calu3 cells, a respiratory cell line, varies slightly and could indicate differences between proteolytic cleavage of the spike proteins between the 2 viruses because of a predicted insertion of a furin cleavage site in SARS-CoV-2 (16). However, differences in cell type and conditions complicate this interpretation and indicate the need for further study in equivalent systems. Overall, the protein expression data from SARS-CoV nucleocapsid and spike protein antibodies recapitulate replication findings and indicate that SARS-CoV reagents can be used to characterize SARS-CoV-2 infection.
Thumbnail of Multistep growth curve for severe acute respiratory syndrome coronavirus 2 from patient with 2019 novel coronavirus disease, United States, 2020. Vero CCL81 (black) and HUH7.0 cells (green) were infected at a multiplicity of infection of 0.1, and cells (solid line) and supernatants (dashed line) were harvested and assayed for viral replication by using TCID50. Circles, Vero CCL81 cells; squares, Vero CCL81 supernatants; triangles, HUH7.0 cells; inverted triangles, HUH7.0 supernatant

Figure 4. Multistep growth curve for severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States, 2020. Vero CCL81 (black) and HUH7.0 cells (green) were infected at a multiplicity of...
Finally, we evaluated the replication kinetics of SARS-CoV-2 in a multistep growth curve. In brief, we infected Vero CCL-81 and HUH7.0 cells with SARS-CoV-2 at a low multiplicity of infection (0.1) and evaluated viral replication every 6 h for 72 h postinoculation, with separate harvests in the cell-associated and supernatant compartments (Figure 4). Similar to SARS-CoV, SARS-CoV-2 replicated rapidly in Vero cells after an initial eclipse phase, achieving 105TCID50/mL by 24 h postinfection and peaking at >106 TCID50/mL. We observed similar titers in cell-associated and supernatant compartments, which indicated efficient egress. Despite peak viral titers by 48 h postinoculation, major CPE was not observed until 60 h postinoculation and peaked at 72 h postinoculation, indicating that infected monolayers should be harvested before peak CPE is observed. Replication in HUH7.0 cells also increased quickly after an initial eclipse phase but plateaued by 24 h postinoculation in the intracellular compartment at 2 × 103 TCID50/mL and decreased after 66 h postinoculation. Virus was not detected in the supernatant of infected HUH7 cells until 36 h postinoculation and exhibited lower titers at all timepoints (Figure 4). Major CPE was never observed in HUH7.0 cells. These results are consistent with previous reports for SARS-CoV and MERS-CoV, which suggested similar replication dynamics between the zoonotic CoV strains (23,24).
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Discussion
We have deposited information on the SARS-CoV-2 USA-WA1/2020 viral strain described here into the Biodefense and Emerging Infections Research Resources Repository (https://www.beiresources.orgExternal Link) reagent resources (American Type Culture Collection, https://www.atcc.orgExternal Link) and the World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch (https://www.utmb.edu/wrcevaExternal Link), to serve as the SARS-CoV-2 reference strain for the United States. The SARS-CoV-2 fourth passage virus has been sequenced and maintains a nucleotide sequence identical to that of the original clinical strain from the United States. These deposits make this virus strain available to the domestic and international public health, academic, and pharmaceutical sectors for basic research, diagnostic development, antiviral testing, and vaccine development. We hope broad access will expedite countermeasure development and testing and enable a better understanding of the transmissibility and pathogenesis of this novel emerging virus.
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Dr. Harcourt is a microbiologist in the National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA. Her research interests are emerging coronavirus replication and antibody responses.
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Acknowledgments
We thank Mavanur R. Suresh for providing plasmid pBM302, which expresses the SARS-CoV nucleocapsid protein.
The reagent described is available through the Biodefense and Emerging Infections Research Resources Repository, National Institutes of Allergy and Infectious Diseases, National Institutes of Health: SARS-related coronavirus 2, isolate USA-WA1/2020, NR-52281.
This study was supported by grants from the National Institute on Aging and the National Institutes of Allergy and Infectious Diseases of the National Institutes of Health (U19AI100625 and R00AG049092 to V.D.M., R24AI120942 to S.C.W., and AI99107 and AI114657 to S.M.); a STARs Award provided by the University of Texas System to V.D.M.; the Institute for Human Infections and Immunity at the University of Texas Medical Branch (S.M.); and trainee funding provided by the McLaughlin Fellowship Fund at the University of Texas Medical Branch.
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DOI: 10.3201/eid2606.200516

Original Publication Date: March 11, 2020

1These authors contributed equally to this article.
2These senior authors contributed equally to this article.
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Volume 26, Number 6—June 2020
Research

Severe Acute Respiratory Syndrome Coronavirus 2 from Patient with Coronavirus Disease, United States
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Jennifer Harcourt1, Azaibi Tamin1, Xiaoyan Lu, Shifaq Kamili, Senthil K. Sakthivel, Janna Murray, Krista Queen, Ying Tao, Clinton R. Paden, Jing Zhang, Yan Li, Anna Uehara, Haibin Wang, Cynthia Goldsmith, Hannah A. Bullock, Lijuan Wang, Brett Whitaker, Brian Lynch, Rashi Gautam, Craig Schindewolf, Kumari G. Lokugamage, Dionna Scharton, Jessica A. Plante, Divya Mirchandani, Steven G. Widen, Krishna Narayanan, Shinji Makino, Thomas G. Ksiazek, Kenneth S. Plante, Scott C. Weaver, Stephen Lindstrom, Suxiang Tong, Vineet D. Menachery2, and Natalie J. Thornburg2Comments to Author
Author affiliations: Centers for Disease Control and Prevention, Atlanta, Georgia, USA (J. Harcourt, A. Tamin, X. Lu, K. Queen, Y. Tao, C.R. Paden, Y. Li, C. Goldsmith, B. Whitaker, R. Gautam, S. Lindstrom, S. Tong, N.J. Thornburg); Eagle Medical Services, Atlanta (S. Kamili, S.K. Sakthivel, J. Murray, B. Lynch); IHRC, Atlanta (J. Zhang, H. Wang); Oak Ridge Institute for Science and Education, Oak Ridge, Tennessee, USA (A. Uehara); Synergy America, Inc., Atlanta (H.A. Bullock, L. Wang); University of Texas Medical Branch, Galveston, Texas, USA (C. Schindewolf, K.G. Lokugamage, D. Mirchandani, S. Widen, K. Narayanan, S. Makino, T.G. Ksiazek, S.C. Weaver, V.D. Menachery); World Reference Center for Emerging Viruses and Arboviruses, Galveston (D. Scharton, J.A. Plante, T.G. Ksiazek, K.S. Plante, S.C. Weaver, V.D. Menachery)
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Abstract
The etiologic agent of an outbreak of pneumonia in Wuhan, China, was identified as severe acute respiratory syndrome coronavirus 2 in January 2020. A patient in the United States was given a diagnosis of infection with this virus by the state of Washington and the US Centers for Disease Control and Prevention on January 20, 2020. We isolated virus from nasopharyngeal and oropharyngeal specimens from this patient and characterized the viral sequence, replication properties, and cell culture tropism. We found that the virus replicates to high titer in Vero-CCL81 cells and Vero E6 cells in the absence of trypsin. We also deposited the virus into 2 virus repositories, making it broadly available to the public health and research communities. We hope that open access to this reagent will expedite development of medical countermeasures.
A novel coronavirus, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), has been identified as the source of a pneumonia outbreak in Wuhan, China, in late 2019 (1,2). The virus was found to be a member of the β coronavirus family, in the same species as SARS-CoV and SARS-related bat CoVs (3,4). Patterns of spread indicate that SARS-CoV-2 can be transmitted person-to-person, and may be more transmissible than SARS-CoV (57). The spike protein of coronaviruses mediates virus binding and cell entry. Initial characterization of SARS-CoV-2 spike indicates that it binds the same receptor as SARS-CoV angiotensin-converting enzyme, which is expressed in both upper and lower human respiratory tracts (8).
The unprecedented rapidity of spread of this outbreak represents a critical need for reference reagents. The public health community requires viral lysates to serve as diagnostic references, and the research community needs virus isolates to test antiviral compounds, develop new vaccines, and perform basic research. In this article, we describe isolation of SARS-CoV-2 from a patient who had coronavirus disease (COVID-19) in the United States and described its genomic sequence and replication characteristics. We have made the virus isolate available to the public health community by depositing it into 2 virus reagent repositories.
Methods
Specimen Collection
Virus isolation from patient samples was deemed not to be human subjects research by the National Center for Immunizations and Respiratory Diseases, Centers for Disease Control and Prevention (CDC) (research determination no. 0900f3eb81ab4b6e). Clinical specimens from a case-patient who had acquired COVID-19 during travel to China and who was identified in Washington, USA, were collected as described (1). Nasopharyngeal (NP) and oropharyngeal (OP) swab specimens were collected on day 3 postsymptom onset, placed in 2–3 mL of viral transport medium, used for molecular diagnosis, and frozen. Confirmed PCR-positive specimens were aliquoted and refrozen until virus isolation was initiated.
Cell Culture, Limiting Dilution, and Virus Isolation
We used Vero CCL-81 cells for isolation and initial passage. We cultured Vero E6, Vero CCL-81, HUH 7.0, 293T, A549, and EFKB3 cells in Dulbecco minimal essential medium (DMEM) supplemented with heat-inactivated fetal bovine serum (5% or 10%) and antibiotics/antimycotics (GIBCO, https://www.thermofisher.comExternal Link). We used both NP and OP swab specimens for virus isolation. For isolation, limiting dilution, and passage 1 of the virus, we pipetted 50 μL of serum-free DMEM into columns 2–12 of a 96-well tissue culture plate, then pipetted 100 μL of clinical specimens into column 1 and serially diluted 2-fold across the plate. We then trypsinized and resuspended Vero cells in DMEM containing 10% fetal bovine serum, 2× penicillin/streptomycin, 2× antibiotics/antimycotics, and 2× amphotericin B at a concentration of 2.5 × 105 cells/mL. We added 100 μL of cell suspension directly to the clinical specimen dilutions and mixed gently by pipetting. We then grew the inoculated cultures in a humidified 37°C incubator in an atmosphere of 5% CO2 and observed for cytopathic effects (CPEs) daily. We used standard plaque assays for SARS-CoV-2, which were based on SARS-CoV and Middle East respiratory syndrome coronavirus (MERS-CoV) protocols (9,10).
When CPEs were observed, we scraped cell monolayers with the back of a pipette tip. We used 50 μL of viral lysate for total nucleic acid extraction for confirmatory testing and sequencing. We also used 50 μL of virus lysate to inoculate a well of a 90% confluent 24-well plate.
Inclusivity/Exclusivity Testing
From the wells in which CPEs were observed, we performed confirmatory testing by using real-time reverse transcription PCR (CDC) and full-genome sequencing (1). The CDC molecular diagnostic assay targets 3 portions of the nucleocapsid gene, and results for all 3 portions must be positive for a sample to be considered positive (https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-detection-instructions.html and https://www.cdc.gov/coronavirus/2019-ncov/lab/rt-pcr-panel-primer-probes.html). To confirm that no other respiratory viruses were present, we performed Fast Track Respiratory Pathogens 33 Testing (FTD Diagnostics, http://www.fast-trackdiagnostics.comExternal Link).
Whole-Genome Sequencing
We designed 37 pairs of nested PCRs spanning the genome on the basis of the coronavirus reference sequence (GenBank accession no. NC045512). We extracted nucleic acid from isolates and amplified by using the 37 individual nested PCRs. We used positive PCR amplicons individually for subsequent Sanger sequencing and also pooled them for library preparation by using a ligation sequencing kit (Oxford Nanopore Technologies, https://nanoporetech.comExternal Link), subsequently for Oxford Nanopore MinION sequencing. We generated consensus nanopore sequences by using Minimap version 2.17 (https://github.comExternal Link) and Samtools version 1.9 (http://www.htslib.orgExternal Link). We generated consensus sequences by Sanger sequencing from both directions by using Sequencher version 5.4.6 (https://www.genecodes.comExternal Link), and further confirmed them by using consensus sequences generated from nanopore sequencing.
To sequence passage 4 stock, we prepared libraries for sequencing by using the Next Ultra II RNA Prep Kit (New England Biolabs, https://www.neb.comExternal Link) according to the manufacturer’s protocol. In brief, we fragmented ≈70–100 ng of RNA for 15 min, followed by cDNA synthesis, end repair, and adaptor ligation. After 6 rounds of PCR, we analyzed libraries by using an Agilent Bioanalyzer (https://www.agilent.comExternal Link) and quantified them by using a quantitative PCR. We pooled samples and sequenced samples by using a paired-end 75-base protocol on an Illumina (Illumina, Inc., https://www.illumina.comExternal Link) MiniSeq instrument and using the High-Output Kit and then processed reads by using Trimmomatic version 0.36 (11) to remove low-quality base calls and any adaptor sequences. We used the de novo assembly program ABySS (12) to assemble the reads into contigs by using several different sets of reads and kmer values ranging from 20 to 40. We compared contigs >400 bases against the National Center for Biotechnology Information (Bethesda, MD, USA) nucleotide collection using BLAST (https://blast.ncbi.nlm.nih.govExternal Link). A nearly full-length viral contig obtained in each sample had 100% identity to the 2019-nCoV/USA-WA1/2020 strain (GenBank accession no. MN985325.1). All the remaining contigs mapped to either host cell rRNA or mitochondria. We mapped the trimmed reads to the reference sequence by using BWA version 0.7.17 (13) and visualized these reads by using the Integrated Genomics Viewer (14) to confirm the identity with the USA-WA1/2020 strain.
Electron Microscopy
We scraped infected Vero cells from the flask, pelleted by low-speed centrifugation, rinsed with 0.1 mol/L phosphate buffer, pelleted again, and fixed for 2 h in 2.5% buffered glutaraldehyde. We then postfixed specimens with 1% osmium tetroxide, en bloc stained with 4% uranyl acetate, dehydrated, and embedded in epoxy resin. We cut ultrathin sections, stained them with 4% uranyl acetate and lead citrate, and examined them by using a Thermo Fisher/FEI Tecnai Spirit electron microscope (https://www.fei.comExternal Link).
Protein Analysis and Western Blotting
We harvested cell lysates by using Laemmli sodium dodecyl sulfate–polyacrylamide gel electrophoresis sample buffer (Bio-Rad, https://www.bio-rad.comExternal Link) containing 2% SDS and 5% β-mercaptoethanol. We removed the cell lysates from a Biosafety Level 3 Laboratory, boiled them, and load them onto a polyacrylamide gel. We subjected the lysates to sodium dodecyl sulfate–polyacrylamide gel electrophoresis, followed by transfer to a polyvinylidene difluoride polyvinylidene fluoride membrane. We then blocked the membrane in 5% nonfat dry milk dissolved in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h, followed by a short wash with TBS-T. We incubated the membrane overnight with primary antibody, either rabbit polyclonal serum against the SARS-CoV spike protein (#40150-T52; Sino Biological, https://www.sinobiological.comExternal Link), β-actin antibody (#4970; Cell Signaling Technology, https://www.cellsignal.comExternal Link), or a custom rabbit polyclonal serum against SARS-CoV nucleocapsid. We then washed the membrane with 3 times with TBS-T and applied horseradish peroxidase-conjugated secondary antibody for 1 h. Subsequently, we washed the membrane 3 times with TBS-T, incubated with Clarity Western ECL Substrate (#1705060S; Bio-Rad), and imaged with a multipurpose imaging system.
Generation of SARS-CoV Nucleocapsid Antibodies
We used the plasmid pBM302 (15) to express SARS-CoV nucleocapsid protein, with a C-terminal His6 tag, to high levels within the inclusion bodies of Escherichia coli and the recombinant protein was purified from the inclusion bodies by using nickel-affinity column chromatography under denaturing conditions. We used stepwise dialysis against Tris/phosphate buffer to refold the recombinant SARS-CoV nucleocapsid protein with decreasing concentrations of urea to renature the protein. We then immunized rabbits with the renatured, full-length, SARS-CoV nucleocapsid protein to generate an affinity-purified rabbit anti–SARS-CoV nucleocapsid protein polyclonal antibody.
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Results
Thumbnail of Cytopathic effect caused by severe acute respiratory syndrome coronavirus 2 from patient with 2019 novel coronavirus disease, United States, 2020. A–C) Phase-contrast microscopy of Vero cell monolayers at 3 days postinoculation: A) Mock, B) nasopharyngeal specimen, C) oropharyngeal specimen. Original magnifications ×10). D) Electron microscopy of virus isolate showing extracellular spherical particles with cross-sections through the nucleocapsids (black dots). Arrow indicates a coro

Figure 1. Cytopathic effect caused by severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States, 2020. A–C) Phase-contrast microscopy of Vero cell monolayers at 3 days postinoculation: A) Mock,...
A patient was identified with confirmed COVID-19 in Washington State on January 22, 2020. CPE was not observed in mock infected cells (Figure 1, panel A). Cycle threshold (Ct) values were 18–20 for NP specimens and 21–22 for OP specimens (1). The positive clinical specimens were aliquoted and refrozen inoculated into cell culture on January 22, 2020. We observed CPE 2 days postinoculation and harvested viral lysate on day 3 postinoculation (Figure 1, panels B, C). We used 50 μL of passage 1 viral lysates for nucleic acid extraction to confirm the presence of SARS-CoV-2 by using the CDC molecular diagnostic assay (1). The Ctvalues of 3 nucleic acid extractions were 16.0–17.1 for nucleocapsid portion 1, 15.9–17.1 for nucleocapsid portion 2, and 16.2–17.3 for nucleocapsid portion 3, which confirmed isolation of SARS-CoV-2 (Ct <40 is considered a positive result). We also tested extracts for 33 additional different respiratory pathogens by using the Fast Track 33 Assay. No other pathogens were detected. Identity was additionally supported by thin-section electron microscopy (Figure 1, panel D). We observed a morphology and morphogenesis characteristic of coronaviruses.
We used isolates from the first passage of an OP and an NP specimen for whole-genome sequencing. The genomes from the NP specimen (GenBank accession MT020880) and OP specimen (GenBank accession no. MT020881) showed 100% identity with each other. The isolates also showed 100% identity with the corresponding clinical specimen (GenBank accession no. MN985325).
After the second passage, we did not culture OP and NP specimens separately. We passaged virus isolate 2 more times in Vero CCL-81 cells and titrated by determining the 50% tissue culture infectious dose (TCID50). Titers were 8.65 × 106 TCID50/mL for the third passage and 7.65 × 106 TCID50/mL for the fourth passage.
We passaged this virus in the absence of trypsin. The spike protein sequence of SARS-CoV-2 has an RRAR insertion at the S1-S2 interface that might be cleaved by furin (16). Highly pathogenic avian influenza viruses have highly basic furin cleavage sites at the hemagglutinin protein HA1-HA2 interface that permit intracellular maturation of virions and more efficient viral replication (17). The RRAR insertion in SARS-CoV-2 might serve a similar function.
Thumbnail of Viral propagation and quantitation of severe acute respiratory syndrome coronavirus 2 from patient with 2019 novel coronavirus disease, United States, 2020. A) Two virus passage 4 stocks (black and gray circles) were quantified by using plaque assay at day 2 (solid circles) and day 3 (open circles) postinfection of Vero E6 and Vero CCL81 cells. B) Plaque morphology for virus on Vero E6 and Vero CCL81 at day 2 and day 3 postinoculation. C) Cell monolayers 2 days postinfection of Vero

Figure 2. Viral propagation and quantitation of severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States, 2020. A) Two virus passage 4 stocks (black and gray circles) were quantified...
We subsequently generated a fourth passage stock of SARS-CoV-2 on VeroE6 cells, another fetal rhesus monkey kidney cell line. We sequenced viral RNA from SARS-CoV-2 passage 4 stock and confirmed it to have no nucleotide mutations compared with the original reference sequence (GenBank accession no. MN985325). SARS-CoV has been found to grow well on VeroE6 cells and MERS-CoV on Vero CCL81 cells (18,19). To establish a plaque assay and determine the preferred Vero cell type for quantification, we titered our passage 4 stock on VeroE6 and VeroCCL81 cells. After infection with a dilution series, SARS-CoV-2 replicated in both Vero cell types; however, the viral titers were slightly higher in VeroE6 cells than in Vero CCL81 cells (Figure 2, panel A). In addition, plaques were more distinct and visible on Vero E6 cells (Figure 2, panel B). As early as 2 days postinoculation, VeroE6 cells produced distinct plaques visible by staining with neutral red. In contrast, Vero CCL81 cells produced less clear plaques and was most easily quantitated by staining with neutral red 3 days postinoculation. On the individual plaque monolayers, SARS-CoV-2 infection of Vero E6 cells produced CPE with areas of cell clearance (Figure 2, panel C). In contrast, Vero CCL81 cells had areas of dead cells that had fused to form plaques, but the cells did not clear. Together, these results suggest that VeroE6 cells might be the best choice for amplification and quantification, but both Vero cell types support amplification and replication of SARS-CoV-2.
Thumbnail of Cell lines from patient with coronavirus disease, United States, 2020, susceptible to SARS coronavirus 2 (SARS-CoV-2). Cell lines were infected with a high multiplicity of infection (>5), washed after adsorption, and subsequently harvested 24 h postinfection for viral titer and protein lysates. A) Viral titer for SARS-CoV-2 quantitated by plaque assay on Vero E6 cells 2 days postinoculation. Infected cell protein lysates were probed by using Western blotting with B) rabbit polycl

Figure 3. Cell lines from patient with coronavirus disease, United States, 2020, susceptible to SARS coronavirus 2 (SARS-CoV-2). Cell lines were infected with a high multiplicity of infection (>5), washed after adsorption, and...
Because research has been initiated to study and respond to SARS-CoV-2, information about cell lines and types susceptible to infection is needed. Therefore, we examined the capacity of SARS-CoV-2 to infect and replicate in several common primate and human cell lines, including human adenocarcinoma cells (A549), human liver cells (HUH7.0), and human embryonic kidney cells (HEK-293T), in addition to Vero E6 and Vero CCL81 cells. We also examined an available big brown bat kidney cell line (EFK3B) for SARS-CoV-2 replication capacity. Each cell line was inoculated at high multiplicity of infection and examined 24 h postinfection (Figure 3, panel A). No CPE was observed in any of the cell lines except in Vero cells, which grew to >107 PFU at 24 h postinfection. In contrast, HUH7.0 and 293T cells showed only modest viral replication, and A549 cells were incompatible with SARS-CoV-2 infection. These results are consistent with previous susceptibility findings for SARS-CoV and suggest other common culture systems, including MDCK, HeLa, HEP-2, MRC-5 cells, and embryonated eggs, are unlikely to support SARS-CoV-2 replication (2022). In addition, SARS-CoV-2 did not replicate in bat EFK3B cells, which are susceptible to MERS-CoV. Together, the results indicate that SARS-CoV-2 maintains a similar profile to SARS-CoV in terms of susceptible cell lines.
Having established robust infection with SARS-CoV-2 in several cell types, we next evaluated the cross-reactivity of SARS-CoV antibodies against the SARS-CoV-2. Cell lysates from infected cell lines were probed for protein analysis; we found that polyclonal serum against the SARS-CoV spike protein and nucleocapsid proteins recognize SARS-CoV-2 (Figure 3, panels B, C). The nucleocapsid protein, which is highly conserved across the group 2B family, retains >90% amino acid identity between SARS-CoV and SARS-CoV-2. Consistent with the replication results (Figure 3, panel A), SARS-CoV-2 showed robust nucleocapsid protein in both Vero cell types, less protein in HUH7.0 and 293T cells, and minimal protein in A549 and EFK3B cells (Figure 3, panel B). The SARS-CoV spike protein antibody also recognized SARS-CoV-2 spike protein, indicating cross-reactivity (Figure 3, panel C). Consistent with SARS CoV, several cleaved and uncleaved forms of the SARS-CoV-2 spike protein were observed. The cleavage pattern of the SARS spike positive control from Calu3 cells, a respiratory cell line, varies slightly and could indicate differences between proteolytic cleavage of the spike proteins between the 2 viruses because of a predicted insertion of a furin cleavage site in SARS-CoV-2 (16). However, differences in cell type and conditions complicate this interpretation and indicate the need for further study in equivalent systems. Overall, the protein expression data from SARS-CoV nucleocapsid and spike protein antibodies recapitulate replication findings and indicate that SARS-CoV reagents can be used to characterize SARS-CoV-2 infection.
Thumbnail of Multistep growth curve for severe acute respiratory syndrome coronavirus 2 from patient with 2019 novel coronavirus disease, United States, 2020. Vero CCL81 (black) and HUH7.0 cells (green) were infected at a multiplicity of infection of 0.1, and cells (solid line) and supernatants (dashed line) were harvested and assayed for viral replication by using TCID50. Circles, Vero CCL81 cells; squares, Vero CCL81 supernatants; triangles, HUH7.0 cells; inverted triangles, HUH7.0 supernatant

Figure 4. Multistep growth curve for severe acute respiratory syndrome coronavirus 2 from patient with coronavirus disease, United States, 2020. Vero CCL81 (black) and HUH7.0 cells (green) were infected at a multiplicity of...
Finally, we evaluated the replication kinetics of SARS-CoV-2 in a multistep growth curve. In brief, we infected Vero CCL-81 and HUH7.0 cells with SARS-CoV-2 at a low multiplicity of infection (0.1) and evaluated viral replication every 6 h for 72 h postinoculation, with separate harvests in the cell-associated and supernatant compartments (Figure 4). Similar to SARS-CoV, SARS-CoV-2 replicated rapidly in Vero cells after an initial eclipse phase, achieving 105TCID50/mL by 24 h postinfection and peaking at >106 TCID50/mL. We observed similar titers in cell-associated and supernatant compartments, which indicated efficient egress. Despite peak viral titers by 48 h postinoculation, major CPE was not observed until 60 h postinoculation and peaked at 72 h postinoculation, indicating that infected monolayers should be harvested before peak CPE is observed. Replication in HUH7.0 cells also increased quickly after an initial eclipse phase but plateaued by 24 h postinoculation in the intracellular compartment at 2 × 103 TCID50/mL and decreased after 66 h postinoculation. Virus was not detected in the supernatant of infected HUH7 cells until 36 h postinoculation and exhibited lower titers at all timepoints (Figure 4). Major CPE was never observed in HUH7.0 cells. These results are consistent with previous reports for SARS-CoV and MERS-CoV, which suggested similar replication dynamics between the zoonotic CoV strains (23,24).
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Discussion
We have deposited information on the SARS-CoV-2 USA-WA1/2020 viral strain described here into the Biodefense and Emerging Infections Research Resources Repository (https://www.beiresources.orgExternal Link) reagent resources (American Type Culture Collection, https://www.atcc.orgExternal Link) and the World Reference Center for Emerging Viruses and Arboviruses, University of Texas Medical Branch (https://www.utmb.edu/wrcevaExternal Link), to serve as the SARS-CoV-2 reference strain for the United States. The SARS-CoV-2 fourth passage virus has been sequenced and maintains a nucleotide sequence identical to that of the original clinical strain from the United States. These deposits make this virus strain available to the domestic and international public health, academic, and pharmaceutical sectors for basic research, diagnostic development, antiviral testing, and vaccine development. We hope broad access will expedite countermeasure development and testing and enable a better understanding of the transmissibility and pathogenesis of this novel emerging virus.
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Dr. Harcourt is a microbiologist in the National Center for Immunization and Respiratory Diseases, Centers for Disease Control and Prevention, Atlanta, GA. Her research interests are emerging coronavirus replication and antibody responses.
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Acknowledgments
We thank Mavanur R. Suresh for providing plasmid pBM302, which expresses the SARS-CoV nucleocapsid protein.
The reagent described is available through the Biodefense and Emerging Infections Research Resources Repository, National Institutes of Allergy and Infectious Diseases, National Institutes of Health: SARS-related coronavirus 2, isolate USA-WA1/2020, NR-52281.
This study was supported by grants from the National Institute on Aging and the National Institutes of Allergy and Infectious Diseases of the National Institutes of Health (U19AI100625 and R00AG049092 to V.D.M., R24AI120942 to S.C.W., and AI99107 and AI114657 to S.M.); a STARs Award provided by the University of Texas System to V.D.M.; the Institute for Human Infections and Immunity at the University of Texas Medical Branch (S.M.); and trainee funding provided by the McLaughlin Fellowship Fund at the University of Texas Medical Branch.
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  7. Chan JF, Yuan S, Kok KH, To KK, Chu H, Yang J, et al. A familial cluster of pneumonia associated with the 2019 novel coronavirus indicating person-to-person transmission: a study of a family cluster. Lancet. 2020;395:514–23. DOIExternal LinkPubMedExternal Link
  8. Wan Y, Shang J, Graham R, Baric RS, Li F. Receptor recognition by novel coronavirus from Wuhan: An analysis based on decade-long structural studies of SARS. J Virol. 2020;JVI.00127-20; Epub ahead of print. DOIExternal LinkPubMedExternal Link
  9. Sims AC, Tilton SC, Menachery VD, Gralinski LE, Schäfer A, Matzke MM, et al. Release of severe acute respiratory syndrome coronavirus nuclear import block enhances host transcription in human lung cells. J Virol. 2013;87:3885–902. DOIExternal LinkPubMedExternal Link
  10. Josset L, Menachery VD, Gralinski LE, Agnihothram S, Sova P, Carter VS, et al. Cell host response to infection with novel human coronavirus EMC predicts potential antivirals and important differences with SARS coronavirus. MBio. 2013;4:e00165–13. DOIExternal LinkPubMedExternal Link
  11. Bolger AM, Lohse M, Usadel B. Trimmomatic: a flexible trimmer for Illumina sequence data.Bioinformatics. 2014;30:2114–20. DOIExternal LinkPubMedExternal Link
  12. Simpson JT, Wong K, Jackman SD, Schein JE, Jones SJ, Birol I. ABySS: a parallel assembler for short read sequence data. Genome Res. 2009;19:1117–23. DOIExternal LinkPubMedExternal Link
  13. Li H, Durbin R. Fast and accurate short read alignment with Burrows-Wheeler transform. Bioinformatics. 2009;25:1754–60. DOIExternal LinkPubMedExternal Link
  14. Robinson JT, Thorvaldsdóttir H, Winckler W, Guttman M, Lander ES, Getz G, et al. Integrative genomics viewer. Nat Biotechnol. 2011;29:24–6. DOIExternal LinkPubMedExternal Link
  15. Das D, Suresh MR. Copious production of SARS-CoV nucleocapsid protein employing codon optimized synthetic gene. J Virol Methods. 2006;137:343–6. DOIExternal LinkPubMedExternal Link
  16. Coutard B, Valle C, de Lamballerie X, Canard B, Seidah NG, Decroly E. The spike glycoprotein of the new coronavirus 2019-nCoV contains a furin-like cleavage site absent in CoV of the same clade. Antiviral Res. 2020;176:104742. DOIExternal LinkPubMedExternal Link
  17. Stieneke-Gröber A, Vey M, Angliker H, Shaw E, Thomas G, Roberts C, et al. Influenza virus hemagglutinin with multibasic cleavage site is activated by furin, a subtilisin-like endoprotease. EMBO J. 1992;11:2407–14. DOIExternal LinkPubMedExternal Link
  18. Li W, Moore MJ, Vasilieva N, Sui J, Wong SK, Berne MA, et al.Angiotensin-converting enzyme 2 is a functional receptor for the SARS coronavirus. Nature. 2003;426:450–4. DOIExternal LinkPubMedExternal Link
  19. Chan JF, Chan KH, Choi GK, To KK, Tse H, Cai JP, et al. Differential cell line susceptibility to the emerging novel human betacoronavirus 2c EMC/2012: implications for disease pathogenesis and clinical manifestation. J Infect Dis. 2013;207:1743–52. DOIExternal LinkPubMedExternal Link
  20. Gillim-Ross L, Taylor J, Scholl DR, Ridenour J, Masters PS, Wentworth DE. Discovery of novel human and animal cells infected by the severe acute respiratory syndrome coronavirus by replication-specific multiplex reverse transcription-PCR.J Clin Microbiol. 2004;42:3196–206. DOIExternal LinkPubMedExternal Link
  21. Kaye M, Druce J, Tran T, Kostecki R, Chibo D, Morris J, et al. SARS-associated coronavirus replication in cell lines. Emerg Infect Dis. 2006;12:128–33. DOIExternal LinkPubMedExternal Link
  22. Swayne DE, Suarez DL, Spackman E, Tumpey TM, Beck JR, Erdman D, et al. Domestic poultry and SARS coronavirus, southern China. Emerg Infect Dis. 2004;10:914–6. DOIExternal LinkPubMedExternal Link
  23. Scobey T, Yount BL, Sims AC, Donaldson EF, Agnihothram SS, Menachery VD, et al. Reverse genetics with a full-length infectious cDNA of the Middle East respiratory syndrome coronavirus. Proc Natl Acad Sci U S A. 2013;110:16157–62. DOIExternal LinkPubMedExternal Link
  24. Yount B, Curtis KM, Fritz EA, Hensley LE, Jahrling PB, Prentice E, et al. Reverse genetics with a full-length infectious cDNA of severe acute respiratory syndrome coronavirus. Proc Natl Acad Sci U S A. 2003;100:12995–3000. DOIExternal LinkPubMedExternal Link
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DOI: 10.3201/eid2606.200516

Original Publication Date: March 11, 2020

1These authors contributed equally to this article.
2These senior authors contributed equally to this article.
Table of Contents – Volume 26, Number 6—June 2020


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Page created: May 18, 2020
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The conclusions, findings, and opinions expressed by authors contributing to this journal do not necessarily reflect the official position of the U.S. Department of Health and Human Services, the Public Health Service, the Centers for Disease Control and Prevention, or the authors' affiliated institutions. Use of trade names is for identification only and does not imply endorsement by any of the groups named above.
Notice the use of non fat dry milk and horseradish in these isolation explanations...I'm now convinced
 

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Microchip Can Detect COVID Before You’re Sick


STORY AT-A-GLANCE
  • Pentagon scientists and Profusa have developed a tiny biosensor that can be embedded under your skin to detect disease
  • Its purpose is to track chemical reactions going on inside your body, which may reveal that you’re infected with a virus like COVID-19 or influenza and about to start having symptoms the next day
  • In addition to the under-skin sensor, the U.S. Defense Advanced Research Projects Agency (DARPA) has been working on a customized filter that can be put on a standard dialysis machine to remove COVID-19 from the blood
  • Profusa said it intended to seek FDA approval for their tissue-integrating biosensor in 2021, and a DARPA-backed study is also underway to measure early signs of influenza via the biosensor technology
  • The biosensors may detect disease outbreaks, biological attacks and pandemics up to three weeks earlier than current methods, but you may have to give up your privacy in exchange

In the "60 Minutes" clip above, Bill Whitaker speaks with Dr. Matt Hepburn, a retired Army infectious disease physician, about a tiny sensor that can be embedded under your skin. Its purpose is to track chemical reactions going on inside your body, which may reveal that you're infected with a virus like COVID-19 or influenza and about to start having symptoms the next day.
Hepburn describes it as a "check engine light,"1 which could have tremendous usefulness, for instance, on an aircraft carrier where thousands of sailors live in close quarters. If the sensor gives the signal that you're "sick," even though you have no symptoms, a blood draw could be self-administered, giving you a diagnosis in three to five minutes.
"As you truncate that time, as you diagnose and treat, what you do is you stop the infection in its tracks," Hepburn said.2 Admittedly, a sensor that's implanted under your skin has an Orwellian ring to it, which is why Whitaker made the disclaimer, "It's not some dreaded government microchip to track your every move, but a tissue-like gel engineered to continuously test your blood."
But in light of the government's recent intrusions on personal liberties and ability to force quarantines on anyone in the name of public safety, even in the absence of illness, isn't that essentially the same thing?
Vaccine Coordinator for Operation Warp Speed
To put this into perspective, consider that Hepburn is the vaccine coordinator for Operation Warp Speed (OWS). OWS, a joint operation between U.S. Health and Human Services (HHS) and the Department of Defense (DOD), continues to be shrouded in secrecy but, little by little, information is emerging that long-term monitoring of the U.S. public is part of the plan.
At face value, OWS is a public-private partnership that was tasked with producing therapeutics and a fast-tracked COVID-19 vaccine.3 OWS invested an estimated $18 billion primarily in late-stage clinical development and early manufacturing of COVID-19 vaccines, and agreements to purchase at least 455 million doses were made.4
Rather than just ensuring a vaccine is produced and made available for those who want it, however, Moncef Slaoui, the chief scientific adviser for Operation Warp Speed — he's been dubbed the coronavirus vaccine czar5 — said in an interview with The Wall Street Journal in October 2020 that the rollout would include "incredibly precise … tracking systems."6,7
Their purpose? "To ensure that patients each get two doses of the same vaccine and to monitor them for adverse health effects."8 In an interview with The New York Times, Slaoui described it as a "very active pharmaco vigilance surveillance system."9
Similar language was reiterated in an October 2020 perspective article published in The New England Journal of Medicine (NEJM), written by Slaoui and Hepburn.10 Writing in NEJM, the duo wrote, "Because some technologies have limited previous data on safety in humans, the long-term safety of these vaccines will be carefully assessed using pharmacovigilance surveillance strategies."11
In addition to working with OWS, Hepburn is a former program manager for the U.S. Defense Advanced Research Projects Agency (DARPA), where he oversaw the development of the implantable biosensor shown in the "60 Minutes" clip with its maker, Profusa.12 The sensor allows a person's physiology to be examined at a distance via smartphone connectivity. Profusa is also backed by Google, the largest data mining company in the world.

Click here to learn more
Is Military Leadership and Total Surveillance the Plan?
OWS, rather than being directed by public health officials, is heavily dominated by military, technology companies and U.S. intelligence agencies, likening it to a successor for Total Information Awareness (TIA), a program managed by DARPA that sprung up after the 9/11 attacks.
At the time, TIA was seeking to collect Americans' medical records, fingerprints and other biometric data, along with DNA and records relating to personal finances, travel and media consumption.13
Hepburn has praised the DOD's role in OWS, calling it "transformative." "One of the most important lessons learned is the value of military leadership," he said during a speech to the Association of Military Surgeons of the United States' virtual annual meeting in December 2020.14
In addition to the vaccine contracts the DOD obtained "in record time that was mutually beneficial" for the vaccine manufacturers, Hepburn told members of the health care community "'to convey a message that these vaccines are safe and efficacious, and that vaccination is important' as a counterpoint to widespread misinformation in the general public about vaccines …"15
Rather than taking over a public health initiative, as it may first appear, Hepburn said the DOD's role in the pandemic was a collaboration not only for Americans but for people globally, and it's set to become the new standard: "[T]his is the new standard for rapid product development, and will apply not only to pandemics but also to develop product for combat health in half the time," he said.16
Stopping Pandemics Before They Begin?
In addition to the under-skin sensor, DARPA has been working on other projects, including a customized filter that can be put on a standard dialysis machine to remove COVID-19 from the blood.
As blood passes through the machine, the virus is removed, returning only healthy blood back to the body. A critically ill spouse of a military member, known only as "Patient 16," reportedly received the treatment for four days and made a full recovery.17
Other scientists have recovered human antibodies for the 1918 Spanish flu, which they got from people still alive today who had lived through that pandemic. When they infected animals with the 1918 flu virus — yes, they still have it — the antibodies were effective in stopping it.18
Hepburn and his team have also funded research on a simulated Zika virus outbreak, creating a cure in 78 days, while other Pentagon researchers are in the process of creating a vaccine that would work against all coronaviruses, even the common cold. It's currently in clinical trials.19
Injectable Biosensor Seeking FDA Approval
Hydrogel is a DARPA invention that involves nanotechnology and nanobots. This "bioelectronic interface" is part of the COVID-19 mRNA vaccines' delivery system. The biochip being developed by Profusa is similar to the proposed COVID-19 mRNA vaccines in that it utilizes hydrogel.
The implant is the size of a grain of rice, and connects to an online database that will keep track of changes in your biochemistry and a wide range of biometrics, such as heart and respiratory rate and much more.
The technology consists of three components:20 the implanted sensor, a reader placed on the surface of the skin and the software that allows the reader to send the collected data via Bluetooth to your phone or tablet, which in turn can be connected to other online sources such as your doctor's website. As Defense One explained in March 2020:21
"The sensor has two parts. One is a 3mm string of hydrogel, a material whose network of polymer chains is used in some contact lenses and other implants. Inserted under the skin with a syringe, the string includes a specially engineered molecule that sends a fluorescent signal outside of the body when the body begins to fight an infection.
The other part is an electronic component attached to the skin. It sends light through the skin, detects the fluorescent signal and generates another signal that the wearer can send to a doctor, website, etc. It's like a blood lab on the skin that can pick up the body's response to illness before the presence of other symptoms, like coughing."
Profusa said it intended to seek FDA approval for their tissue-integrating biosensor in 2021,22 and a DARPA-backed study is also underway to measure early signs of influenza via the biosensor technology. The injectable sensors will be used to measure physiological statuses to reveal not only indicators of human response to infection but also "exposure to disease in healthy volunteers."23
A wireless patch that measures tissue oxygen levels would also be used, sending information to a mobile device for real-time data. According to Profusa, the biosensors may detect disease outbreaks, biological attacks and pandemics up to three weeks earlier than current methods.24 It would seem, however, that in order for such sensors to work on a widespread scale, extensive adoption would be required.
24-Hour Monitoring in Exchange for 'Safety'
There are glaring privacy and ethical concerns when it comes to rolling out an implantable sensor that will track your every sniffle, even before you reach for a tissue. The information will then be sent digitally to your cellphone, and who will have access? Perhaps an even worse prospect is, what information could potentially be sent the other way — from the sensor into your body? For instance, technology critic Adam Keiper pointed out in The New Atlantis:25
"Aside from nanotech's potential as a weapon of mass destruction, it could also make possible totally novel forms of violence and oppression. Nanotechnology could theoretically be used to make mind-control systems, invisible and mobile eavesdropping devices, or unimaginably horrific tools of torture."
In order to stop a disease outbreak three weeks early, offering a fearful public an illusion of safety, you'd only have to give up your privacy, and submit to being monitored and hooked up to "the cloud," perhaps permanently.
If you remember TIA after the 9/11 attacks, you may also remember that it was quickly defunded by Congress after significant public backlash, including concerns that TIA would undermine personal privacy.
In the case of OWS and the emerging biosensors, there's little negative press, and media outlets are overwhelmingly supportive of the operation as a way to resolve the COVID-19 crisis and future pandemics. One of my favorite independent journalists, Whitney Webb, put it this way:
"It's certainly alarming, and it seems to point to the fulfillment of an agenda that was attempted to be pushed through or foisted on the American public after 9/11, called Total Information Awareness, which was managed, originally, by DARPA.
It was about using medical data and non-medical data — essentially all data about you — to prevent terror attacks before they could happen, and also to prevent bioterror attacks and even prevent naturally occurring disease outbreaks.
A lot of the same initiatives proposed under that original program after 9/11 have essentially been resurrected, with updated technology, under the guise of combating COVID-19."
 

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t
7,766 DEAD 330,218 Injuries: European Database of Adverse Drug Reactions for COVID-19 “Vaccines”

April 24, 2021
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7,766 DEAD 330,218 Injuries: European Database of Adverse Drug Reactions for COVID-19 “Vaccines”





by Brian Shilhavy
Editor, Health Impact News

The European database of suspected drug reaction reports is EudraVigilance, which also tracks reports of injuries and deaths following the experimental COVID-19 “vaccines.”
Here is what EudraVigilance states about their database:
This website was launched by the European Medicines Agency in 2012 to provide public access to reports of suspected side effects (also known as suspected adverse drug reactions). These reports are submitted electronically to EudraVigilance by national medicines regulatory authorities and by pharmaceutical companies that hold marketing authorisations (licences) for the medicines.
EudraVigilance is a system designed for collecting reports of suspected side effects. These reports are used for evaluating the benefits and risks of medicines during their development and monitoring their safety following their authorisation in the European Economic Area (EEA). EudraVigilance has been in use since December 2001.
This website was launched to comply with the EudraVigilance Access Policy, which was developed to improve public health by supporting the monitoring of the safety of medicines and to increase transparency for stakeholders, including the general public.
The Management Board of the European Medicines Agency first approved the EudraVigilance Access Policy in December 2010. A revision was adopted by the Board in December 2015 based on the 2010 pharmacovigilance legislation. The policy aims to provide stakeholders such as national medicines regulatory authorities in the EEA, the European Commission, healthcare professionals, patients and consumers, as well as the pharmaceutical industry and research organisations, with access to reports on suspected side effects.
Transparency is a key guiding principle of the Agency, and is pivotal to building trust and confidence in the regulatory process. By increasing transparency, the Agency is better able to address the growing need among stakeholders, including the general public, for access to information. (Source.)
Their report through April 17, 2021 lists 7,766 deaths and 330,218 injuries following injections of four experimental COVID-19 shots:
A Health Impact News subscriber in Europe ran the reports for each of the four COVID-19 shots we are including here. This subscriber has volunteered to do this, and it is a lot of work to tabulate each reaction with injuries and fatalities, since there is no place on the EudraVigilance system we have found that tabulates all the results.
Here is the summary data through April 17, 2021.
Total reactions for the experimental mRNA vaccine Tozinameran (code BNT162b2, Comirnaty) from BioNTech/ Pfizer: 4,293 deaths and 144,607 injuries to 17/04/2021
 

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Biologists 'transfer' a memory through RNA injection
Research in marine snails could lead to new treatments to restore memories and alter traumatic ones
Date:May 14, 2018Source:University of California - Los AngelesSummary:Biologists report they have transferred a memory from one marine snail to another, creating an artificial memory, by injecting RNA from one to another. This research could lead to new ways to treat traumatic memories with RNA -- perhaps a traumatic memory could be altered -- and perhaps new ways to restore lost memories.
 

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Jew scientism has altered the code in a corn plant to not die when glysophates are poured on it.

Jew science wants to alter your code so they can pour something on you and you won't exactly die, you merely shuffle on and comply...

cRispr and other "editing" technocracies are advancing faster than mere plebes will know.

If DARPA is just now announcing thier "help" in fighting covid911, you can bet it's been decades in the labs and test kitchens already.

Nobody warped speeded anything, this eugenics shit been in the pipe along time.
 

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ICAN, through its attorneys, has once again written to Dr. Rochelle Walensky, the new Director of the CDC, along with Peter Marks, director of the Center for Biologics Evaluation and Research, this time to demand that the FDA and CDC confirm whether or not they still maintain that COVID-19 vaccines cannot change human DNA.

On March 16, 2021, ICAN’s attorneys wrote to Dr. Walensky and Dr. Marks to inquire about viral vector COVID-19 vaccines and the CDC’s assertion that “[t]he genetic material delivered by the viral vector does not integrate into a person’s DNA.”
ICAN pointed out a recent study which explains that “studies have shown that replication-incompetent adenoviral vectors randomly integrate into host chromosomes at frequencies of 0.001-1% of infected cells” and asked that Dr. Walensky and Dr. Marks either provide the science to explain how these studies are incorrect or confirm removal of CDC’s contrary assertion.
On March 22, 2021, we received a two-sentence reply email from Sandra Cashman thanking us for our letter and our “interest in COVID-19 vaccines and viral vector technology” and directing us to “see the latest information on the COVID-19 response at https://www.cdc.gov/COVID-19/.” This site also states that “COVID-19 vaccines do not change or interact with your DNA in any way.”
We, therefore, wrote again to follow-up on that request and to also bring the following study to their attention which indicates that segments of SARS-CoV-2 viral RNA can become integrated into human genomic DNA and that this newly acquired viral sequence is not silent, meaning that these genetically modified regions of genomic DNA are transcriptionally active (DNA is being converted back into RNA).
Due to the apparent inconsistencies, we asked that Drs. Walensky and Marks advise whether the CDC and FDA still maintain that it is not possible for the mRNA in the COVID-19 vaccines to integrate into a vaccinee’s DNA.
ICAN continues to ask the hard questions of Dr. Walensky and others. ICAN will closely review any response from Dr. Walensky or Dr. Marks and will continue to push for improved transparency regarding vaccines and their development, clinical trials, and safety surveillance.


You can share this legal update via this link: www.icandecide.org/
 

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The Vaccine Is Experimental Gene Therapy
You cannot have a “vaccine” that does not meet the definition of a vaccine. Up until recently, Merriam-Webster defined a vaccine as “a preparation of killed microorganisms, living attenuated organisms, or living fully virulent organisms that is administered to produce or artificially increase immunity to a particular disease.”2

COVID-19 vaccines are not conventional vaccines made with live or attenuated viruses. They're actually gene therapies. The Pfizer and Moderna vaccines are made with lipid nanoparticles that contain polyethylene glycol (PEG)3 and messenger RNA (mRNA). mRNA are snippets of genetic code that carries instructions for cells to produce proteins.

The definition of genetic is “relating to genes” and genes contain instructional code that tell the body what proteins to make. Therapy is the medical treatment of disease, so mRNA vaccines are very clearly gene therapy. In fact, in Moderna’s 2018 SEC filing, Moderna states: “Currently, mRNA is considered a gene therapy product by the FDA.”4

Another problem is related to how long the mRNA remains stable in your system, as it’s encased in a nanolipid to prevent it from degrading too rapidly.

The idea behind mRNA vaccines is that by tricking your body into creating the SARS-CoV-2 spike protein, your immune system will produce antibodies in response. But what happens when you turn your body into a viral protein factory, thus keeping antibody production activated on a continual basis with no ability to shut down?

_______________________________________________________________________________________________________________________________________________________________

monsanto can edit a plant to act a certain way when a certain pathogen interacts with it.

big pharma believes your body can be entertained within this same or similar paradigm.

your God given systems are nothing more to rockefeller medicine than a chit to be extracted as a lifelong customer and captured servant.

you should be edited at birth with a vit k injection, a hep b injection and 100 more edits to your system by the time you might luckily reach age if consent or 18.

the human as it stands today in the western world soaked up to its eyeballs in medicines and edits, can't reproduce like it once could, it can't produce the same vitality of sperm or eggs as it once could.

the human of today is over half its number inflicted with chronic metabolic diseases, even as children the numbers are staggering.

this isn't because humans forget how to reproduce or live ealthy lives, this is because of the lifestyles and destruction of families and rockefeller medicine regimes. its because we are seperated from the logos of the universe, we are separated from God.

we are seperated from the natural order of the universe with God as the focal point in our directions.

man knows better what your systems should have for a healthy life? not showing much progress towards that end.

soils are degraded, water is degraded, air is degraded, the family is degraded, the sexes are degraded, public discourse is degraded, life itself is in full degraded mode and running headlong into an abyss.

God is in charge of history, EMJones like to say that it might takes disasters and calamities to bring about changes in attitudes and changes in beliefs and changes in history. shits got to get really disastrously bad and at some point before an all encompassing destruction, the maddness stops and a better life emerges.

like an recovering drug addict or alcaholic, you don't know you are in it or want to change it until you hit the rock bottom or worst human you can be, before you ever want to change to be better man you sometimes need to be the worst man you can be...how would you even know what a better man was if you were not a disaster.

on this individual level you could see how we get to these points in history, we probably are not even in the worst part of a shift yet?

it could get worse.

of course now is not the time to lose faith...


Mark 4:35-41

King James Version


35 And the same day, when the even was come, he saith unto them, Let us pass over unto the other side.
36 And when they had sent away the multitude, they took him even as he was in the ship. And there were also with him other little ships.
37 And there arose a great storm of wind, and the waves beat into the ship, so that it was now full.
38 And he was in the hinder part of the ship, asleep on a pillow: and they awake him, and say unto him, Master, carest thou not that we perish?
39 And he arose, and rebuked the wind, and said unto the sea, Peace, be still. And the wind ceased, and there was a great calm.
40 And he said unto them, Why are ye so fearful? how is it that ye have no faith?
41 And they feared exceedingly, and said one to another, What manner of man is this, that even the wind and the sea obey him?


in a dark hour as any hour, I'm going to put my faith in the resurrection and healing powers of Jesus.

you too could heal your mind through faith in the risen Christ.

they cannot win

we are assured of life through Christ, It makes all the days from this one to the last so very fruitful and joyous.

get Some!
 

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do we "shed" exosomes and parts of ourselves?

I dunno, what's science say about that?

if the science nigger is telling you to be afraid of the unvaxxed because he might "shed" unwanted something something at you..what's to stop the vaxxed from shedding something something detrimental onto you? hmmmmm science muffagas!?

_______________________________________________________________________________________________________________________________________________________________


Shedding microvesicles: artefacts no more
E Cocucci, G Racchetti, J Meldolesi - Trends in cell biology, 2009 - Elsevier
The small vesicles shed from the surface of many cells upon stimulation, considered for a
long time to be artefacts, are now recognized as specific structures that are distinct from the
exosomes released upon exocytosis of multivesicular bodies. Recent reports indicate that …
 

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From the way back file


https://www.bitchute.com/channel/pcs/

A second interview with Dee Nicholson on "The Liquid Lunch" show courtesy of ThatChannel.comregarding Vaccines, the threat of Mandatory Vaccinations and Bill C-6 (Canada). Contains some more up to date information and the latest developments.Recorded on October 6th, 2009.
This is a 30 minute excerpt from the 2 hour long "Liquid Lunch Show", hosted by Randy Thomas and Co-host Lisa Small in Toronto, Canada, which features a variety of guests and topics. It airs live each Tuesday, via streaming video at www.ThatChannel.com (The regular host is Hugh Reilly)
https://rumble.com/vgdbs5-2009-talk-on-vaccines-talks-about-staged-pandemic-and-depopulation.html
 
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Escaped True Master

Crsper. And dreds
 

anti-barabas-ite

Work stuff through in your brain...UNVAXXED
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Escaped True Master
OK new thought experiment:

The vaccine is nothing because the flu was nothing.

Vaccine isn't going to kill everybody or alter their genome, right now.

The plot is to shut everything off as hard as they dare using flu , old people that die anyways die harder perhaps. Flu shots and continued mutations of flu's keep lock downs going.

Rebuilding from the chaos that they might still be willing to engineer.

Everyone at one level is in on it, the top .0005%

They rest are schmos. And expendable.

It's going to be rebuilt, china, russia anglo american oligarchs are In on it.

My question remains , how hard do they push for a conflagration that will need to be rebuilt from.

Is it just crushing "representative democracy"?

America really isn't in the way as a corpration.

But white debbils just might be, the newest terrorists. White male anti vaxxers...

I'm going to think on this plot some more...
 

anti-barabas-ite

Work stuff through in your brain...UNVAXXED
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🐸 Citizen of the Internet 🐸
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Escaped True Master
Dr. Lee Merritt with mike Adam's health ranger nutters.


Yeah squaline mf23.

Get some.

Spike protien shakes, get some
 
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